caa a22 4500 606157832 CHVBK 20210128100615.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10096-014-2229-1 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2229-1 High levels of CD4+ CTLA-4+ Treg cells and CCR5 density in HIV-1-infected patients with visceral leishmaniasis [Elektronische Daten] [A. Vallejo, M. Abad-Fernández, S. Moreno, A. Moreno, M. Pérez-Elías, F. Dronda, J. Casado] Visceral leishmaniasis (VL) in HIV-1-infected patients has been associated with poor immunological recovery and frequent disease relapses. The aim of this study was to analyse the role of T cell populations, Treg cells and CCR5 density in patients with VL compared to HIV-1-infected patients without leishmaniasis. A cross-sectional study of nine Leishmania-HIV-1-coinfected (LH) patients with VL receiving suppressive cART for at least 1year were compared to 16 HIV-1-infected patients with non-immunological response (NIR, CD4 count below 250 cells/mm3) and 26 HIV-1-infected patients with immunological response (IR, CD4 count above 500 cells/mm3) without leishmaniasis. LH patients had a deep depletion of naïve T cells (p = 0.002), despite similar levels of effector T cells compared to NIR patients. CD4 Treg cells were similar compared to NIR patients, but higher compared to IR patients (p < 0.001). Interestingly, CD4 Treg CTLA-4+ cells were higher in LH patients compared to either NIR or IR patients (p = 0.022 and p < 0.001, respectively), and the CD4 Treg/TEM ratio was similar to NIR patients, but higher compared to IR patients (p = 0.017). CCR5+ T cell levels were higher compared to IR patients (p < 0.001), while CCR5 density on T cells were higher compared to both NIR and IR patients (p < 0.005 in both cases). Higher levels of CD4+ CTLA-4+ Treg cells and CCR5 density on CD8+ T cells are strongly associated with VL in HIV-1-infected patients. Also, these patients have a poor immunological profile that might explain the persistence and relapse of the pathogen. Springer-Verlag Berlin Heidelberg, 2014 Vallejo A. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut Abad-Fernández M. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut Moreno S. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut Moreno A. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut Pérez-Elías M. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut Dronda F. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut Casado J. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 267-275 0934-9723 34:2<267 2015 34 10096 https://doi.org/10.1007/s10096-014-2229-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2229-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vallejo A. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Abad-Fernández M. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Moreno S. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Moreno A. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Pérez-Elías M. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Dronda F. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut NATIONALLICENCE 700 1- Casado J. Department of Infectious Diseases, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), University Hospital Ramón y Cajal, Ctra Colmenar Km 9, 28034, Madrid, Spain aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 267-275 0934-9723 34:2<267 2015 34 10096