caa a22 4500 606157921 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10096-014-2240-6 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2240-6 Species distribution and susceptibility profile to fluconazole, voriconazole and MXP-4509 of 551 clinical yeast isolates from a Romanian multi-centre study [Elektronische Daten] [B. Minea, V. Nastasa, R. Moraru, A. Kolecka, M. Flonta, I. Marincu, A. Man, F. Toma, M. Lupse, B. Doroftei, N. Marangoci, M. Pinteala, T. Boekhout, M. Mares] This is the first multi-centre study regarding yeast infections in Romania. The aim was to determine the aetiological spectrum and susceptibility pattern to fluconazole, voriconazole and the novel compound MXP-4509. The 551 isolates were identified using routine laboratory methods, matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and DNA sequence analysis. Susceptibility testing was performed using the European Committee for Antimicrobial Susceptibility Testing (EUCAST) method and breakpoints. The yeasts originated from superficial infections (SUP, 51.5%), bloodstream infections (BSI, 31.6%) and deep-seated infections (DEEP, 16.9%), from patients of all ages. Nine genera and 30 species were identified. The 20 Candida species accounted for 94.6% of all isolates. C. albicans was the overall leading pathogen (50.5%). Lodderomyces elongisporus is reported for the first time as a fungaemia cause in Europe. C. glabrata and Saccharomyces cerevisiae, as well as the non-Candida spp. and non-albicans Candida spp. groups, showed decreased fluconazole susceptibility (<75%). The overall fluconazole resistance was 10.2%. C. krusei accounted for 27 of the 56 fluconazole-resistant isolates. The overall voriconazole resistance was 2.5% and was due mainly to C. glabrata and C. tropicalis isolates. Fluconazole resistance rates for the three categories of infection were similar to the overall value; voriconazole resistance rates differed: 4% for BSI, 3.2% for DEEP and 1.4% for SUP. The antifungal activity of MXP-4509 was superior to voriconazole against C. glabrata and many fluconazole-resistant isolates. There was a large percentage of non-albicans Candida isolates. A large part of the high fluconazole resistance was not acquired but intrinsic, resulting from the high percentage of C. krusei. Springer-Verlag Berlin Heidelberg, 2014 Minea B. Advanced Research Centre for Bionanoconjugates and Biopolymers, Institute of Macromolecular Chemistry "Petru Poni”, 700487, Iasi, Romania aut Nastasa V. Laboratory of Antimicrobial Chemotherapy, "Ion Ionescu de la Brad” University, 8 Aleea Sadoveanu, P4, 1st floor, 700489, Iasi, Romania aut Moraru R. Laboratory of Antimicrobial Chemotherapy, "Ion Ionescu de la Brad” University, 8 Aleea Sadoveanu, P4, 1st floor, 700489, Iasi, Romania aut Kolecka A. Yeast and Basidiomycete Research, CBS Fungal Biodiversity Centre (CBS-KNAW), 3508 AD, Utrecht, The Netherlands aut Flonta M. Microbiology Laboratory, Clinical Hospital of Infectious Diseases, 400348, Cluj-Napoca, Romania aut Marincu I. Department of Infectious Diseases, "Victor Babes” University of Medicine and Pharmacy, 300041, Timisoara, Romania aut Man A. Department of Microbiology, Virology and Parasitology, University of Medicine and Pharmacy, 540139, Tirgu Mures, Romania aut Toma F. Department of Microbiology, Virology and Parasitology, University of Medicine and Pharmacy, 540139, Tirgu Mures, Romania aut Lupse M. Department of Infectious Diseases, "Iuliu Hatieganu” University of Medicine and Pharmacy, 400012, Cluj-Napoca, Romania aut Doroftei B. 2nd Clinic of Obstetrics and Gynaecology, "Gr. T. Popa” University of Medicine and Pharmacy, 700115, Iasi, Romania aut Marangoci N. Advanced Research Centre for Bionanoconjugates and Biopolymers, Institute of Macromolecular Chemistry "Petru Poni”, 700487, Iasi, Romania aut Pinteala M. Advanced Research Centre for Bionanoconjugates and Biopolymers, Institute of Macromolecular Chemistry "Petru Poni”, 700487, Iasi, Romania aut Boekhout T. Yeast and Basidiomycete Research, CBS Fungal Biodiversity Centre (CBS-KNAW), 3508 AD, Utrecht, The Netherlands aut Mares M. Laboratory of Antimicrobial Chemotherapy, "Ion Ionescu de la Brad” University, 8 Aleea Sadoveanu, P4, 1st floor, 700489, Iasi, Romania aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 367-383 0934-9723 34:2<367 2015 34 10096 https://doi.org/10.1007/s10096-014-2240-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2240-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Minea B. Advanced Research Centre for Bionanoconjugates and Biopolymers, Institute of Macromolecular Chemistry "Petru Poni”, 700487, Iasi, Romania aut NATIONALLICENCE 700 1- Nastasa V. Laboratory of Antimicrobial Chemotherapy, "Ion Ionescu de la Brad” University, 8 Aleea Sadoveanu, P4, 1st floor, 700489, Iasi, Romania aut NATIONALLICENCE 700 1- Moraru R. Laboratory of Antimicrobial Chemotherapy, "Ion Ionescu de la Brad” University, 8 Aleea Sadoveanu, P4, 1st floor, 700489, Iasi, Romania aut NATIONALLICENCE 700 1- Kolecka A. Yeast and Basidiomycete Research, CBS Fungal Biodiversity Centre (CBS-KNAW), 3508 AD, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Flonta M. Microbiology Laboratory, Clinical Hospital of Infectious Diseases, 400348, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Marincu I. Department of Infectious Diseases, "Victor Babes” University of Medicine and Pharmacy, 300041, Timisoara, Romania aut NATIONALLICENCE 700 1- Man A. Department of Microbiology, Virology and Parasitology, University of Medicine and Pharmacy, 540139, Tirgu Mures, Romania aut NATIONALLICENCE 700 1- Toma F. Department of Microbiology, Virology and Parasitology, University of Medicine and Pharmacy, 540139, Tirgu Mures, Romania aut NATIONALLICENCE 700 1- Lupse M. Department of Infectious Diseases, "Iuliu Hatieganu” University of Medicine and Pharmacy, 400012, Cluj-Napoca, Romania aut NATIONALLICENCE 700 1- Doroftei B. 2nd Clinic of Obstetrics and Gynaecology, "Gr. T. Popa” University of Medicine and Pharmacy, 700115, Iasi, Romania aut NATIONALLICENCE 700 1- Marangoci N. Advanced Research Centre for Bionanoconjugates and Biopolymers, Institute of Macromolecular Chemistry "Petru Poni”, 700487, Iasi, Romania aut NATIONALLICENCE 700 1- Pinteala M. Advanced Research Centre for Bionanoconjugates and Biopolymers, Institute of Macromolecular Chemistry "Petru Poni”, 700487, Iasi, Romania aut NATIONALLICENCE 700 1- Boekhout T. Yeast and Basidiomycete Research, CBS Fungal Biodiversity Centre (CBS-KNAW), 3508 AD, Utrecht, The Netherlands aut NATIONALLICENCE 700 1- Mares M. Laboratory of Antimicrobial Chemotherapy, "Ion Ionescu de la Brad” University, 8 Aleea Sadoveanu, P4, 1st floor, 700489, Iasi, Romania aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 367-383 0934-9723 34:2<367 2015 34 10096