caa a22 4500 606157948 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10096-014-2228-2 doi (NATIONALLICENCE)springer-10.1007/s10096-014-2228-2 Propionibacterium acnes populations involved in deep pathological samples and their dynamics along the cardiac surgical pathway [Elektronische Daten] [S. Romano-Bertrand, M. Beretta, H. Jean-Pierre, J.-M. Frapier, B. Calvet, S. Parer, E. Jumas-Bilak] Propionibacterium acnes belongs to the normal skin microbiota, but it is also responsible for acne vulgaris and causes serious infections such as endocarditis and surgical site infections (SSI). The P. acnes population is structured into phylogenetic groups, with phylotype I being associated with acne. Herein, we explore the link between phylotypes and clinical origins in a collection of P. acnes isolated from different body sites, involved in deep infections or healthcare-associated infections (HAI), with particular emphasis on strains from cardiac SSI. Cardiac SSI have been further studied in terms of P. acnes population dynamics during the care pathway. The recA and tly genes phylotypes were compared to hemolytic behavior, susceptibility to antimicrobial agents, and clinical origins. An original approach of recA polymerase chain reaction temporal temperature gel electrophoresis (PCR-TTGE) was developed and applied for the direct identification of P. acnes phylotypes in surgical samples, in order to assess their temporal dynamics during the surgical course. Our results underlined the preferential involvement of IA-2/IB and II phylogroups in HAI and SSI. Unlike IA and II, type IA-2/IB presented a gradual increase with the depth of sampling in the peroperative phase of cardiac surgery. Phylotypes IA and IA-2/IB were both predominant in scar tissues and on postoperative skin, suggesting a specific predisposition to recolonize skin. Particular association of the phylotype IA-2/IB with SSI and its propensity to colonize wounds in cardiac surgery was observed. We assumed that the follow-up of P. acnes phylotypes during pathological processes could give new clues for P. acnes pathogenicity. Springer-Verlag Berlin Heidelberg, 2014 Romano-Bertrand S. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut Beretta M. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut Jean-Pierre H. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut Frapier J.-M Cardio-thoracic Surgery Unit, University Hospital of Montpellier, Montpellier, France aut Calvet B. Cardio-thoracic Intensive Care Unit, University Hospital of Montpellier, Montpellier, France aut Parer S. Bacteriology Laboratory, University Hospital of Montpellier, Montpellier, France aut Jumas-Bilak E. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 287-301 0934-9723 34:2<287 2015 34 10096 https://doi.org/10.1007/s10096-014-2228-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-014-2228-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Romano-Bertrand S. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Beretta M. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Jean-Pierre H. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 700 1- Frapier J.-M Cardio-thoracic Surgery Unit, University Hospital of Montpellier, Montpellier, France aut NATIONALLICENCE 700 1- Calvet B. Cardio-thoracic Intensive Care Unit, University Hospital of Montpellier, Montpellier, France aut NATIONALLICENCE 700 1- Parer S. Bacteriology Laboratory, University Hospital of Montpellier, Montpellier, France aut NATIONALLICENCE 700 1- Jumas-Bilak E. UMR 5119 ECOSYM, Equipe Pathogènes et Environnements, U.F.R. des Sciences Pharmaceutiques et Biologiques, Université Montpellier 1, 15, Avenue Charles Flahault, BP 14491, 34093, Montpellier Cedex 5, France aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/2(2015-02-01), 287-301 0934-9723 34:2<287 2015 34 10096