caa a22 4500 606157972 CHVBK 20210128100616.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10096-015-2468-9 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2468-9 Development and evaluation of MALDI-TOF MS-based serotyping for Streptococcus pneumoniae [Elektronische Daten] [S. Nakano, Y. Matsumura, Y. Ito, T. Fujisawa, B. Chang, S. Suga, K. Kato, T. Yunoki, G. Hotta, T. Noguchi, M. Yamamoto, M. Nagao, S. Takakura, M. Ohnishi, T. Ihara, S. Ichiyama] Surveillance of Streptococcus pneumoniae serotypes is important for the successful implementation of vaccination strategies to prevent the spread of invasive pneumococcal diseases. The standard method of serotyping of pneumococcal isolates is the phenotypic Neufeld test, which is cost- and labor-intensive. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been implemented as a rapid, simple and inexpensive method for identifying species. We evaluated the performance of MALDI-TOF MS for serotyping ten major serotypes of S. pneumoniae in Japan (serotypes 3, 6B, 15A, 15C, 19A, 19F, 23A, 24F, 35B and 38) using the Biotyper and ClinProTools. After optimizing the settings, we validated their serotyping performance for serotypes 3, 15A and 19A using a separate set of isolates that were not used in the creation of the classification algorithms. A total of 574 isolates of S. pneumoniae collected from Japanese nationwide surveillance studies were included. Of these, 407 isolates belonged to the ten major serotypes. Biotyper and ClinProTools correctly identified 77.9% and 84.0%, respectively, of the ten major serotype isolates. The validation analysis included a total of 113 isolates of the serotypes 3, 15A and 19A isolates. Biotyper and ClinProTools correctly identified 85.0% and 69.9% of the validation cohort isolates, respectively. MALDI-TOF MS has the potential to discriminate the ten major S. pneumoniae serotypes prevalent in Japan. Springer-Verlag Berlin Heidelberg, 2015 Nakano S. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Matsumura Y. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Ito Y. Department of Respiratory Medicine, Allergy and Rheumatology, Nagoya City University Graduate School of Medical Science, Nagoya, Japan aut Fujisawa T. Department of Clinical Research, National Hospital Organization Mie National Hospital, Tsu, Japan aut Chang B. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan aut Suga S. Department of Clinical Research, National Hospital Organization Mie National Hospital, Tsu, Japan aut Kato K. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Yunoki T. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Hotta G. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Noguchi T. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Yamamoto M. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Nagao M. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Takakura S. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut Ohnishi M. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan aut Ihara T. Department of Paediatrics, National Hospital Organization Mie National Hospital, Tsu, Japan aut Ichiyama S. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/11(2015-11-01), 2191-2198 0934-9723 34:11<2191 2015 34 10096 https://doi.org/10.1007/s10096-015-2468-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2468-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakano S. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Matsumura Y. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Ito Y. Department of Respiratory Medicine, Allergy and Rheumatology, Nagoya City University Graduate School of Medical Science, Nagoya, Japan aut NATIONALLICENCE 700 1- Fujisawa T. Department of Clinical Research, National Hospital Organization Mie National Hospital, Tsu, Japan aut NATIONALLICENCE 700 1- Chang B. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan aut NATIONALLICENCE 700 1- Suga S. Department of Clinical Research, National Hospital Organization Mie National Hospital, Tsu, Japan aut NATIONALLICENCE 700 1- Kato K. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Yunoki T. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Hotta G. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Noguchi T. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Yamamoto M. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Nagao M. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Takakura S. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 700 1- Ohnishi M. Department of Bacteriology I, National Institute of Infectious Diseases, Tokyo, Japan aut NATIONALLICENCE 700 1- Ihara T. Department of Paediatrics, National Hospital Organization Mie National Hospital, Tsu, Japan aut NATIONALLICENCE 700 1- Ichiyama S. Department of Clinical Laboratory Medicine, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto, Japan aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/11(2015-11-01), 2191-2198 0934-9723 34:11<2191 2015 34 10096