caa a22 4500 606158103 CHVBK 20210128100617.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10096-015-2475-x doi (NATIONALLICENCE)springer-10.1007/s10096-015-2475-x Routine disc diffusion antimicrobial susceptibility testing of Clostridium difficile and association with PCR ribotype 027 [Elektronische Daten] [H. Holt, T. Danielsen, U. Justesen] Reduced susceptibility to metronidazole and vancomycin in Clostridium difficile has been reported, which emphasises the need for simple antimicrobial susceptibility testing methods. The aim of this study was to apply a published disc diffusion method and zone diameter breakpoint correlates to the European Committee on Antimicrobial Susceptibility Testing (EUCAST) epidemiological minimum inhibitory concentration (MIC) cut-off values in a routine setting. Metronidazole and vancomycin zone diameters from 2702 isolates were recorded. Fifteen isolates had a metronidazole zone diameter below the published breakpoint (<23mm) and five isolates had a vancomycin zone diameter below the published breakpoint (<19mm), most of which were polymerase chain reaction (PCR) ribotype 027. The total number of PCR ribotype 027 was 29 (1.1%). Overall, C. difficile PCR ribotype 027 isolates had smaller zone diameters than non-027 isolates. The disc diffusion method is very simple and inexpensive, and the published zone diameter breakpoints will detect C. difficile isolates with reduced susceptibility to metronidazole and vancomycin. Springer-Verlag Berlin Heidelberg, 2015 Holt H. Department of Clinical Microbiology, Odense University Hospital, Winsloewparken 21, 2, 5000, Odense C, Denmark aut Danielsen T. Department of Clinical Microbiology, Odense University Hospital, Winsloewparken 21, 2, 5000, Odense C, Denmark aut Justesen U. Department of Clinical Microbiology, Odense University Hospital, Winsloewparken 21, 2, 5000, Odense C, Denmark aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/11(2015-11-01), 2243-2246 0934-9723 34:11<2243 2015 34 10096 https://doi.org/10.1007/s10096-015-2475-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2475-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Holt H. Department of Clinical Microbiology, Odense University Hospital, Winsloewparken 21, 2, 5000, Odense C, Denmark aut NATIONALLICENCE 700 1- Danielsen T. Department of Clinical Microbiology, Odense University Hospital, Winsloewparken 21, 2, 5000, Odense C, Denmark aut NATIONALLICENCE 700 1- Justesen U. Department of Clinical Microbiology, Odense University Hospital, Winsloewparken 21, 2, 5000, Odense C, Denmark aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/11(2015-11-01), 2243-2246 0934-9723 34:11<2243 2015 34 10096