caa a22 4500 606158146 CHVBK 20210128100617.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s10096-015-2470-2 doi (NATIONALLICENCE)springer-10.1007/s10096-015-2470-2 Assessment of a molecular diagnostic platform for integrated isolation and quantification of mRNA in whole blood [Elektronische Daten] [C. van den Kieboom, G. Ferwerda, I. de Baere, H. Vermeiren, R. de Groot, R. Rossau, M. de Jonge] Implementation of point-of-care tests may facilitate the health management of infectious diseases by reducing the timeframe on pathogen identification and host response measurements, allowing for immediate diagnosis and guided clinical intervention. In this feasibility study, a novel totally integrated and fully automated real-time polymerase chain reaction (PCR) platform (Idylla™, Biocartis) was assessed to determine the mRNA expression levels of multiple genes from 1mL of whole blood. To this purpose, a sample-in result-out assay, including mRNA extraction and RT-qPCR-based detection, was ported to the platform. The genes used (matrix metallopeptidase 9, olfactomedin 4, NB1 glycoprotein and lipocalin 2) were previously identified as predictive for severity of disease caused by infection with respiratory syncytial virus (RSV). The reproducibility and robustness of the prototype assay was determined using the blood samples of 21 healthy donors. The data showed that the Idylla™ platform allows for a fast and user-friendly determination of the relative expression levels of the four selected mRNA markers. The Author(s), 2015 van den Kieboom C. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut Ferwerda G. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut de Baere I. R&D Department, Biocartis N.V., Mechelen, Belgium aut Vermeiren H. R&D Department, Biocartis N.V., Mechelen, Belgium aut de Groot R. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut Rossau R. R&D Department, Biocartis N.V., Mechelen, Belgium aut de Jonge M. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/11(2015-11-01), 2209-2212 0934-9723 34:11<2209 2015 34 10096 https://doi.org/10.1007/s10096-015-2470-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10096-015-2470-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- van den Kieboom C. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Ferwerda G. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- de Baere I. R&D Department, Biocartis N.V., Mechelen, Belgium aut NATIONALLICENCE 700 1- Vermeiren H. R&D Department, Biocartis N.V., Mechelen, Belgium aut NATIONALLICENCE 700 1- de Groot R. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut NATIONALLICENCE 700 1- Rossau R. R&D Department, Biocartis N.V., Mechelen, Belgium aut NATIONALLICENCE 700 1- de Jonge M. Department of Pediatrics, Laboratory of Pediatric Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands aut NATIONALLICENCE 773 0- European Journal of Clinical Microbiology & Infectious Diseases Springer Berlin Heidelberg 34/11(2015-11-01), 2209-2212 0934-9723 34:11<2209 2015 34 10096