caa a22 4500 606161317 CHVBK 20210128100634.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10526-014-9605-8 doi (NATIONALLICENCE)springer-10.1007/s10526-014-9605-8 Identification and characterization of a novel marine Bacillus cereus VCRC-B540 for mosquito control [Elektronische Daten] [Chinnasamy Mani, Krishnaraj Thirugnanasambantham, Somaiah Sundarapandian, Subbiah Poopathi] A marine bacterium with mosquitocidal effect was isolated from the gut region of the marine red snapper fish (Lutjanus sanguineous). The 16S rRNA gene sequence alignment showed that this isolate belonged to the strain Bacillus cereus VCRC-B540 (NCBI: JN377787). Biochemical studies showed that the strain could be useful in mosquito control. It showed an increasing pattern of toxicity for Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti, without negative effects for the non-targeted organisms Chironomus riparius, Daphnia cephalata and Notonecta glauca. A qualitative analysis of B. cereus showed that a polypeptide (M.wt: 90kDa) was associated with the toxicity observed. Consequently, the peptide sequence is identified to be a surface layer protein. A phylogenetic analysis of 16S rDNA gene sequence of B. cereus revealed shared homology with the Bacillus species. Hence, it is concluded that the marine bacterium (B. cereus) can be useful in the biological control of mosquito vectors. International Organization for Biological Control (IOBC), 2014 Bacillus cereus nationallicence Lutjanus sanguineous nationallicence Toxicity assays nationallicence Mosquitoes nationallicence Surface layer protein nationallicence Phylogenetic analysis nationallicence Mani Chinnasamy Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, 605 006, Puducherry, India aut Thirugnanasambantham Krishnaraj Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, 605 006, Puducherry, India aut Sundarapandian Somaiah Department of Ecology and Environmental Sciences, School of Life-Sciences, Pondicherry University, 605014, Puducherry-, India aut Poopathi Subbiah Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, 605 006, Puducherry, India aut BioControl Springer Netherlands 60/1(2015-02-01), 71-79 1386-6141 60:1<71 2015 60 10526 https://doi.org/10.1007/s10526-014-9605-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-014-9605-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mani Chinnasamy Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, 605 006, Puducherry, India aut NATIONALLICENCE 700 1- Thirugnanasambantham Krishnaraj Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, 605 006, Puducherry, India aut NATIONALLICENCE 700 1- Sundarapandian Somaiah Department of Ecology and Environmental Sciences, School of Life-Sciences, Pondicherry University, 605014, Puducherry-, India aut NATIONALLICENCE 700 1- Poopathi Subbiah Unit of Microbiology and Immunology, Vector Control Research Centre (Indian Council of Medical Research), Indira Nagar, 605 006, Puducherry, India aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/1(2015-02-01), 71-79 1386-6141 60:1<71 2015 60 10526