caa a22 4500 606161635 CHVBK 20210128100635.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s10526-014-9644-1 doi (NATIONALLICENCE)springer-10.1007/s10526-014-9644-1 Long-term storage of eggs of Leptoglossus occidentalis for the mass-rearing of its parasitoid Gryon pennsylvanicum [Elektronische Daten] [Giuseppino Sabbatini Peverieri, Paola Furlan, Daniele Benassai, Ward Strong, Pio Roversi] Leptoglossus occidentalis Heidemann (Heteroptera, Coreidae) is a harmful insect to cones and seeds of several conifers in North America, and was accidentally introduced into Europe where it rapidly spread to many countries. In Central Italy, the pest damaged cones and seeds of Pinus pinea L., leading to a severe reduction of pine nut production. Gryon pennsylvanicum (Ashmead) (Hymenoptera, Platygastridae) is the most common egg-parasitoid of L. occidentalis in British Columbia. This natural enemy has been successfully reared under laboratory conditions. However, the possibility to arrange a sufficient large number of specimens is crucial for the success in biological control. In the present work, stored L. occidentalis eggs at fridge and freezing temperatures of +4, −20°C, and ultra-low temperatures of −80 and −140°C for different times up to oneyear, were tested for parasitization success. Results showed that G. pensylvanicum can exploit successfully host eggs stored for long time when stored at ultra-low temperatures and that storage did not affect fitness of the first and second generation. For shorter periods of time, the use of +4°C for store eggs did not vary significantly from eggs stored at ultra-low temperatures. International Organization for Biological Control (IOBC), 2014 Western Conifer Seed Bug nationallicence Classical biological control nationallicence Cold storage nationallicence Egg parasitoid nationallicence Sabbatini Peverieri Giuseppino Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut Furlan Paola Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut Benassai Daniele Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut Strong Ward Kalamalka Forestry Centre, British Columbia Ministry of Forests, Lands and Natural Resource Operations, 3401 Reservoir Rd, V1B 2C7, Vernon, BC, Canada aut Roversi Pio Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut BioControl Springer Netherlands 60/3(2015-06-01), 293-306 1386-6141 60:3<293 2015 60 10526 https://doi.org/10.1007/s10526-014-9644-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-014-9644-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sabbatini Peverieri Giuseppino Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut NATIONALLICENCE 700 1- Furlan Paola Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut NATIONALLICENCE 700 1- Benassai Daniele Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut NATIONALLICENCE 700 1- Strong Ward Kalamalka Forestry Centre, British Columbia Ministry of Forests, Lands and Natural Resource Operations, 3401 Reservoir Rd, V1B 2C7, Vernon, BC, Canada aut NATIONALLICENCE 700 1- Roversi Pio Consiglio per la Ricerca e la sperimentazione in Agricoltura, Centro di Ricerca per l'Agrobiologia e la Pedologia, Via di Lanciola 12/a, 50125, Florence, Italy aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/3(2015-06-01), 293-306 1386-6141 60:3<293 2015 60 10526