caa a22 4500 606161678 CHVBK 20210128100635.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10526-015-9666-3 doi (NATIONALLICENCE)springer-10.1007/s10526-015-9666-3 Genetic and biological characterisation of a novel Plutella xylostella granulovirus, PlxyGV-SA [Elektronische Daten] [Fatima Abdulkadir, Caroline Knox, Tamryn Marsberg, Martin Hill, Sean Moore] Plutella xylostella granulovirus (PlxyGV) has been isolated from insect populations in many countries and is considered a potential biopesticide for sustainable control of P. xylostella (L.) (Lepidoptera: Plutellidae). Several PlxyGV isolates have been genetically characterised, and the full genome sequence of PlxyGV-Japan is available for comparison with novel isolates. A South African PlxyGV was recently recovered from an overcrowded laboratory P. xylostella colony and identified as a genetically distinct isolate by sequencing of the granulin gene and restriction endonuclease (REN) analysis of genomic DNA. In this report, PlxyGV-SA was further characterised by PCR amplification and sequencing of egt, lef-8 and lef-9 genes, and several amino acid substitutions were observed. The PstI REN profile of PlxyGV-SA was different from that of PlxyGV-Japan in terms of band size and number, thereby confirming its novel genetic identity. Surface dose bioassays showed that PlxyGV-SA is pathogenic to neonate but not late instar larvae at the same and higher virus doses, indicating that a biopesticide should be targeted at early larval stages in the field. International Organization for Biological Control (IOBC), 2015 Diamondback moth nationallicence Plutella xylostella nationallicence Granulovirus nationallicence Bioassay nationallicence Abdulkadir Fatima Department of Biochemistry and Microbiology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut Knox Caroline Department of Biochemistry and Microbiology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut Marsberg Tamryn Department of Zoology & Entomology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut Hill Martin Department of Zoology & Entomology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut Moore Sean Department of Zoology & Entomology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut BioControl Springer Netherlands 60/4(2015-08-01), 507-515 1386-6141 60:4<507 2015 60 10526 https://doi.org/10.1007/s10526-015-9666-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-015-9666-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Abdulkadir Fatima Department of Biochemistry and Microbiology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 700 1- Knox Caroline Department of Biochemistry and Microbiology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 700 1- Marsberg Tamryn Department of Zoology & Entomology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 700 1- Hill Martin Department of Zoology & Entomology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 700 1- Moore Sean Department of Zoology & Entomology, Rhodes University, PO Box 94, 6140, Grahamstown, South Africa aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/4(2015-08-01), 507-515 1386-6141 60:4<507 2015 60 10526