caa a22 4500 606161686 CHVBK 20210128100635.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10526-015-9664-5 doi (NATIONALLICENCE)springer-10.1007/s10526-015-9664-5 Intra-individual variability of ITS regions in entomopathogenic nematodes (Steinernematidae: Nematoda): implications for their taxonomy [Elektronische Daten] [Vladimír Půža, Daniela Chundelová, Jiří Nermuť, Martina Žurovcová, Zdeněk Mráček] The internal transcribed spacers of rDNA are among the most important molecular markers used in the taxonomy and phylogeny of entomopathogenic nematodes. However, their ease of use and the results they provide can be confounded by the presence of intra-individual variability. In the present study we screened 40 strains of S. feltiae and S. kraussei and nine strains from the "glaseri” group. In two selected strains of S. feltiae, and one strain each of S. kraussei and S. glaseri, PCR products of four virgin females were cloned, and five clones per individual female were sequenced and analyzed. In S. feltiae, intra-individual variability was compared with intraspecific variability. We revealed a high frequency of intra-individual variability, which reached 65% in S. feltiae, 30% in S. kraussei, and almost 90% in the "glaseri” group. Analysis of the cloned products showed intra-individual variability in each of the tested strains and individuals. Maximum within-individual variability was over 1% in S. feltiae and S. kraussei, and in S. glaseri reached up to 4%. In S. feltiae, intra-individual variability equaled the variability among S. feltiae strains from different parts of the world. Our data show that intra-individual variability in the ITS regions is quite frequent in entomopathogenic nematodes. The implications of this fact to the group taxonomy are discussed. International Organization for Biological Control (IOBC), 2015 Steinernema nationallicence Genetic diversity nationallicence Intra-individual variability nationallicence ITS nationallicence Taxonomy nationallicence Půža Vladimír Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Chundelová Daniela Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Nermuť Jiří Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Žurovcová Martina Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut Mráček Zdeněk Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut BioControl Springer Netherlands 60/4(2015-08-01), 547-554 1386-6141 60:4<547 2015 60 10526 https://doi.org/10.1007/s10526-015-9664-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-015-9664-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Půža Vladimír Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Chundelová Daniela Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Nermuť Jiří Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Žurovcová Martina Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 700 1- Mráček Zdeněk Laboratory of Entomopathogenic nematodes, Institute of Entomology, Biology Centre of the ASCR, Branišovská 31, 370 05, České Budějovice, Czech Republic aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/4(2015-08-01), 547-554 1386-6141 60:4<547 2015 60 10526