caa a22 4500 606161732 CHVBK 20210128100636.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10526-015-9658-3 doi (NATIONALLICENCE)springer-10.1007/s10526-015-9658-3 Differential susceptibility of pine weevil, Hylobius abietis (Coleoptera: Curculionidae), larvae and pupae to entomopathogenic nematodes and death of adults infected as pupae [Elektronische Daten] [Christopher Williams, Aoife Dillon, Darragh Ennis, Roseanne Hennessy, Christine Griffin] The large pine weevil Hylobius abietis is a serious pest of reforestation in northern Europe. Development takes place in the stumps of felled conifer trees and emerging adults feed on and kill newly planted trees. Application of entomopathogenic nematodes around tree stumps has been shown to reduce the emergence of adult weevils. In order to target application at the most susceptible stage, the susceptibility of larvae and pupae to Heterorhabditis downesi and Steinernema carpocapsae was compared in a close-contact assay on filter paper. An average of 95.8% of larvae were killed by H. downesi and 82.1% by S. carpocapsae while only 16.3 and 15.0% of pupae were killed by these two species, respectively. However, many of the H. abietis that were exposed as pupae died after metamorphosis to callow adult, with mortality of pupae and callow adults combined reaching 62.5% for H. downesi and 69.9% for S. carpocapsae. For both nematode species significantly more insects died as larvae than as either pupae or pupae/callow adults. When pupae were exposed to infective juveniles (IJs) for 2days and were then washed while still pupae to remove surface IJs, adults were later found to be infected indicating that IJs can infect pupae, survive metamorphosis and subsequently kill adults. International Organization for Biological Control (IOBC), 2015 Pine weevil nationallicence Entomopathogenic nematodes nationallicence Differential susceptibility nationallicence Forest pests nationallicence Biocontrol nationallicence Williams Christopher Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut Dillon Aoife Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut Ennis Darragh Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut Hennessy Roseanne Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut Griffin Christine Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut BioControl Springer Netherlands 60/4(2015-08-01), 537-546 1386-6141 60:4<537 2015 60 10526 https://doi.org/10.1007/s10526-015-9658-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-015-9658-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Williams Christopher Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut NATIONALLICENCE 700 1- Dillon Aoife Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut NATIONALLICENCE 700 1- Ennis Darragh Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut NATIONALLICENCE 700 1- Hennessy Roseanne Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut NATIONALLICENCE 700 1- Griffin Christine Department of Biology, Maynooth University, Maynooth, Co. Kildare, Ireland aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/4(2015-08-01), 537-546 1386-6141 60:4<537 2015 60 10526