caa a22 4500 606161740 CHVBK 20210128100636.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s10526-015-9655-6 doi (NATIONALLICENCE)springer-10.1007/s10526-015-9655-6 Antifungal activity of Serratia marcescens CFFSUR-B2 purified chitinolytic enzymes and prodigiosin against Mycosphaerella fijiensis , causal agent of black Sigatoka in banana ( Musa spp.) [Elektronische Daten] [Martha Gutiérrez-Román, Francisco Holguín-Meléndez, Michael Dunn, Karina Guillén-Navarro, Graciela Huerta-Palacios] The ascomycete fungus Mycosphaerella fijiensis causes black Sigatoka disease of banana. Because Serratia marcescens strain CFFSUR-B2 is an effective agent for the biological control of M. fijiensis, it was important to determine the mechanisms by which this bacterium stops or inhibits infection by this phytopathogen. The individual chitinases were effective in reducing germ tube growth to different degrees. We evaluated also the effect of mixtures of purified chitinases (ChiA, ChiB and ChiC) and prodigiosin on the germination and germ tube growth of M. fijiensis ascospores. None of the combinations inhibited ascospore germination. However, a toxic effect similar to that of benzimidazole was observed on ascospore germination by the synergistic action of chitinases and prodigiosin applied in combination. The maximal effect in inhibiting germ tube development observed with a mixture of the chitinases and prodigiosin suggests that these could be used in the effective biocontrol of black Sigatoka disease. International Organization for Biological Control (IOBC), 2015 Biocontrol nationallicence Biological interactions nationallicence Chitinases nationallicence Prodigiosin nationallicence Biofungicides nationallicence Sigatoka negra nationallicence Gutiérrez-Román Martha Departamento de Agricultura, Sociedad y Ambiente, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut Holguín-Meléndez Francisco Laboratorio de Diagnóstico Fitosanitario, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut Dunn Michael Programa de Genómica Funcional de Procariotes, Centro de Ciencias Genómicas UNAM, Av. Universidad S/N, Col. Chamilpa, 62210, Cuernavaca, Morelos, Mexico aut Guillén-Navarro Karina Laboratorio de Biotecnología Ambiental y Agroecológica, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut Huerta-Palacios Graciela Departamento de Agricultura, Sociedad y Ambiente, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut BioControl Springer Netherlands 60/4(2015-08-01), 565-572 1386-6141 60:4<565 2015 60 10526 https://doi.org/10.1007/s10526-015-9655-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-015-9655-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gutiérrez-Román Martha Departamento de Agricultura, Sociedad y Ambiente, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut NATIONALLICENCE 700 1- Holguín-Meléndez Francisco Laboratorio de Diagnóstico Fitosanitario, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut NATIONALLICENCE 700 1- Dunn Michael Programa de Genómica Funcional de Procariotes, Centro de Ciencias Genómicas UNAM, Av. Universidad S/N, Col. Chamilpa, 62210, Cuernavaca, Morelos, Mexico aut NATIONALLICENCE 700 1- Guillén-Navarro Karina Laboratorio de Biotecnología Ambiental y Agroecológica, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut NATIONALLICENCE 700 1- Huerta-Palacios Graciela Departamento de Agricultura, Sociedad y Ambiente, El Colegio de la Frontera Sur, Carretera Antiguo Aeropuerto Km. 2.5, 30700, Tapachula, Chiapas, Mexico aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/4(2015-08-01), 565-572 1386-6141 60:4<565 2015 60 10526