caa a22 4500 606161864 CHVBK 20210128100636.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10526-015-9674-3 doi (NATIONALLICENCE)springer-10.1007/s10526-015-9674-3 Chitinolytic activity in Serratia marcescens (strain SEN) and potency against different larval instars of Spodoptera litura with effect of sublethal doses on insect development [Elektronische Daten] [Chetana Aggarwal, Sangeeta Paul, Vishwas Tripathi, Bishwajeet Paul, Md. Khan] Chitinase-producing bacteria were isolated from diseased insect [Pieris brassicae Linnaeus (Lepidoptera: Pieridae) and Spodoptera litura Fabricius (Lepidoptera: Noctuidae)] cadavers. Serratia marcescens strain SEN showed highest enzyme production at temperature 27°C and pH 9.0 and was selected for further studies. It possessed exochitinase, endochitinase and chitobiosidase activities, of which endochitinase was found to be the predominant chitinase. Serratia marcescens strain SEN showed insecticidal activity against all the developmental stages of S. litura larvae. Ingestion of sublethal doses of S. marcescens strain SEN led to a decrease in the larval and pupal weight, percent normal pupation, adult emergence and a significant increase of the larval period. Effect on fecundity and egg hatchability were studied at LC20 and LC50 doses. Fecundity was significantly reduced at both doses tested, while egg hatchability was significantly affected only at the highest dose. To our knowledge, this is the first report of the potential of an entomopathogenic S. marcescens strain against different developmental stages of S. litura. The tested S. marcescens strain SEN showed promise as a biocontrol agent of S. litura. International Organization for Biological Control (IOBC), 2015 Spodoptera litura nationallicence Serratia marcescens nationallicence Chitinase activity nationallicence Sublethal dose nationallicence Insect development nationallicence Aggarwal Chetana Division of Microbiology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Paul Sangeeta Division of Microbiology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Tripathi Vishwas School of Biotechnology, Gautam Buddha University, 201308, Greater Noida, Uttar Pradesh, India aut Paul Bishwajeet Division of Entomology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut Khan Md Department of Biology, Faculty of Science, Jazan University, Jazan, Kingdom of Saudi Arabia aut BioControl Springer Netherlands 60/5(2015-10-01), 631-640 1386-6141 60:5<631 2015 60 10526 https://doi.org/10.1007/s10526-015-9674-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-015-9674-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Aggarwal Chetana Division of Microbiology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- Paul Sangeeta Division of Microbiology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- Tripathi Vishwas School of Biotechnology, Gautam Buddha University, 201308, Greater Noida, Uttar Pradesh, India aut NATIONALLICENCE 700 1- Paul Bishwajeet Division of Entomology, Indian Agricultural Research Institute, 110 012, New Delhi, India aut NATIONALLICENCE 700 1- Khan Md Department of Biology, Faculty of Science, Jazan University, Jazan, Kingdom of Saudi Arabia aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/5(2015-10-01), 631-640 1386-6141 60:5<631 2015 60 10526