caa a22 4500 606161902 CHVBK 20210128100636.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s10526-015-9671-6 doi (NATIONALLICENCE)springer-10.1007/s10526-015-9671-6 Biological control of the European cockchafer: persistence of Beauveria brongniartii after long-term applications in the Euroregion Tyrol [Elektronische Daten] [Johanna Mayerhofer, Jürg Enkerli, Roland Zelger, Hermann Strasser] Melocont® Pilzgerste, a commercial product based on the entomopathogenic fungus Beauveria brongniartii (Sacc.) Petch (Ascomycota: Hypocreales) strain BIPESCO 2, was applied at different rates and time points over a period of 24years in East-, North- and South Tyrol (Austria and Italy) to control the European cockchafer (Melolontha melolontha L. and M. hippocastani F.). Beauveria spp. density was assessed and Beauveria spp. isolates were characterized at species and genotype level using Bloc intergenic region sequence and simple sequence repeat marker analyses. BIPESCO 2 was detected at 41% of the treated sites, predominantly at sites where the fungus was applied at least once during the fouryears prior to sampling. At one site BIPESCO 2 was detected 15years after the last treatment. Results showed that applications should be repeated to achieve good persistence of the biological control agent, and it indicated that despite intensive applications diverse populations of B. brongniartii or Beauveria spp. consortia can coexist in these habitats. International Organization for Biological Control (IOBC), 2015 Mycoinsecticide nationallicence Inoculation biological control nationallicence Hypocreales nationallicence Cordycipitaceae nationallicence Coleoptera nationallicence Scarabaeidae nationallicence Mayerhofer Johanna Institute of Microbiology, Leopold-Franzens University Innsbruck, Technikerstraβe 25, 6020, Innsbruck, Austria aut Enkerli Jürg Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046, Zurich, Switzerland aut Zelger Roland Research Centre for Agriculture and Forestry Laimburg, Laimburg 6, 39040, Ora/Auer, Italy aut Strasser Hermann Institute of Microbiology, Leopold-Franzens University Innsbruck, Technikerstraβe 25, 6020, Innsbruck, Austria aut BioControl Springer Netherlands 60/5(2015-10-01), 617-629 1386-6141 60:5<617 2015 60 10526 https://doi.org/10.1007/s10526-015-9671-6 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-015-9671-6 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mayerhofer Johanna Institute of Microbiology, Leopold-Franzens University Innsbruck, Technikerstraβe 25, 6020, Innsbruck, Austria aut NATIONALLICENCE 700 1- Enkerli Jürg Molecular Ecology, Institute for Sustainability Sciences, Agroscope, Reckenholzstrasse 191, 8046, Zurich, Switzerland aut NATIONALLICENCE 700 1- Zelger Roland Research Centre for Agriculture and Forestry Laimburg, Laimburg 6, 39040, Ora/Auer, Italy aut NATIONALLICENCE 700 1- Strasser Hermann Institute of Microbiology, Leopold-Franzens University Innsbruck, Technikerstraβe 25, 6020, Innsbruck, Austria aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/5(2015-10-01), 617-629 1386-6141 60:5<617 2015 60 10526