caa a22 4500 606161937 CHVBK 20210128100637.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10526-014-9636-1 doi (NATIONALLICENCE)springer-10.1007/s10526-014-9636-1 Searching ISR determinant/s from Bacillus subtilis IAGS174 against Fusarium wilt of tomato [Elektronische Daten] [Waheed Akram, Tehmina Anjum, Basharat Ali] Bacillus subtilis IAGS174 has been previously shown to induce systemic resistance in tomato plants against Fusarium wilt disease. In the present investigation, the resistance-inducing determinant was isolated from cell-free culture filtrates (CFCF) of this bacterium. For this purpose, CFCF was extracted by a series of organic solvents, and the fraction that showed induced systemic resistance (ISR) activity was further partitioned into sub-fractions by column chromatography by using a stepwise elution method. Gas chromatography/mass spectrometry analysis identified four compounds in the ISR-active sub-fraction viz. eugenol, 3-methoxy butyl acetate, pentachloroaniline and phthalic acid methyl ester (PAME). Subsequent bioassays proved that PAME is the potential ISR determinant that significantly ameliorated Fusarium wilt disease of tomato at concentrations of 0.01 and 0.1mM. Furthermore, compared to the respective controls, tomato plants treated with PAME showed increased activities of defense-related enzymes such as phenylalanine ammonia-lyase, polyphenol oxidase, and peroxidase. Our research indicates that B. subtilis IAGS174 has great potential for use as a biological control agent, and PAME is the ISR determinant secreted by this bacterium into the rhizosphere. This determinant can effectively trigger defense responses in tomato plants. International Organization for Biological Control (IOBC), 2014 Induced systemic resistance (ISR) nationallicence B. subtilis IAGS174 nationallicence Tomato nationallicence Fusarium wilt disease nationallicence Phthalic acid methyl ester nationallicence Phenylalanine ammonia-lyase nationallicence Polyphenol oxidase nationallicence Peroxidase nationallicence Akram Waheed Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan aut Anjum Tehmina Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan aut Ali Basharat Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan aut BioControl Springer Netherlands 60/2(2015-04-01), 271-280 1386-6141 60:2<271 2015 60 10526 https://doi.org/10.1007/s10526-014-9636-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-014-9636-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Akram Waheed Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan aut NATIONALLICENCE 700 1- Anjum Tehmina Institute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan aut NATIONALLICENCE 700 1- Ali Basharat Department of Microbiology and Molecular Genetics, University of the Punjab, Lahore, Pakistan aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/2(2015-04-01), 271-280 1386-6141 60:2<271 2015 60 10526