caa a22 4500 606161988 CHVBK 20210128100637.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10526-014-9630-7 doi (NATIONALLICENCE)springer-10.1007/s10526-014-9630-7 Cold tolerance of the predatory ladybird Cryptolaemus montrouzieri [Elektronische Daten] [Sara Maes, Jean-Claude Grégoire, Patrick De Clercq] The effect of low temperature acclimation and diet on the supercooling point (SCP, the temperature at which the insect's body fluids freeze) and lethal time (LTime, time required to kill 50% of the population at a temperature of 5°C) of the mealybug destroyer, Cryptolaemus montrouzieri Mulsant (Coleoptera: Coccinellidae), was assessed in the laboratory. The SCP of acclimated adult ladybirds which were allowed to complete development to adulthood at 18°C and a 8:16(L:D)h photoperiod, or at 25°C and a 16:8(L:D)h photoperiod, and which were subsequently kept at 10°C and a 12:12(L:D)h photoperiod for sevendays, was −17.4 and −16.8°C, respectively. These SCP-values were approximately 7°C lower than the value of −9.9°C for non-acclimated ladybirds maintained at a temperature of 25°C and a photoperiod of 16:8(L:D)h throughout development and in the first week of their adult life. Also food source had a significant effect on the freezing temperature of C. montrouzieri: the SCP of ladybirds fed the citrus mealybug, Planococcus citri (Risso)(Hemiptera: Pseudococcidae), was 1.6°C higher than the value of −17.2°C observed for ladybirds provided with eggs of the flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). However, neither cold acclimation nor diet had a significant effect on the lethal times of C. montrouzieri. Overall, the time required to kill 50% of the population at a temperature of 5°C ranged from 12.8days for ladybirds fed P. citri mealybugs to 14.4days for ladybirds fed E. kuehniella eggs. All individuals exposed to a constant 5°C had died by day 24. Based on the results from this laboratory study, it is deemed unlikely that C. montrouzieri could establish outdoors in western Europe, and it is therefore expected to pose little risk to non-target species in this area when used as an augmentative biological control agent. International Organization for Biological Control (IOBC), 2014 Biological control nationallicence Environmental risk assessment nationallicence Cold tolerance nationallicence Non-native species nationallicence Predator nationallicence Coleoptera nationallicence Coccinellidae nationallicence Maes Sara Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut Grégoire Jean-Claude Biological Control and Spatial Ecology Lab, ULB, 50 Avenue F. D. Roosevelt - CP 160/12, 1050, Brussels, Belgium aut De Clercq Patrick Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut BioControl Springer Netherlands 60/2(2015-04-01), 199-207 1386-6141 60:2<199 2015 60 10526 https://doi.org/10.1007/s10526-014-9630-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-014-9630-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maes Sara Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 700 1- Grégoire Jean-Claude Biological Control and Spatial Ecology Lab, ULB, 50 Avenue F. D. Roosevelt - CP 160/12, 1050, Brussels, Belgium aut NATIONALLICENCE 700 1- De Clercq Patrick Laboratory of Agrozoology, Department of Crop Protection, Ghent University, Coupure Links 653, 9000, Ghent, Belgium aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/2(2015-04-01), 199-207 1386-6141 60:2<199 2015 60 10526