caa a22 4500 606161996 CHVBK 20210128100637.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10526-014-9637-0 doi (NATIONALLICENCE)springer-10.1007/s10526-014-9637-0 Molecular markers for Diadegma (Hymenoptera: Ichneumonidae) species distinction and their use to study the effects of companion plants on biocontrol of the diamondback moth [Elektronische Daten] [Ivan Juric, Walter Salzburger, Henryk Luka, Oliver Balmer] Molecular markers facilitate the quantification of parasitization of pest species and the distinction of related parasitoid species. We designed new markers that allow the distinction of often misidentified Diadegma semiclausum and D. fenestrale parasitization in field-collected diamondback moth (Plutella xylostella) larvae. The markers were applied to study if cornflower (Centaurea cyanus) companion plants increase parasitization of the diamondback moth by Diadegma parasitoids in cabbage fields, as they do in the Mamestra brassicae—Microplitis mediator pest-parasitoid pair. Among 1708 P. xylostella larvae analyzed, we found a high parasitization rate (72.2% total, 41.7% by D. semiclausum, 24.9% by D. fenestrale, 5.6% by both), but no significant effects of cornflower presence. Our results highlight the need for species-specific markers and the specific action of companion plants. To increase natural control in crop fields, habitat management needs to be tailored to—and acts on—specific target species. International Organization for Biological Control (IOBC), 2014 Parasitic wasp nationallicence Mitochondrial COI nationallicence Diagnostic multiplex PCR nationallicence Species-specific marker nationallicence Biological control nationallicence Plutella xylostella nationallicence Diadegma semiclausum nationallicence Diadegma fenestrale nationallicence Juric Ivan Research Institute of Organic Agriculture (FiBL), Ackerstrasse 21, 5070, Frick, Switzerland aut Salzburger Walter Zoological Institute, Evolutionary Biology, University of Basel, Vesalgasse 1, 4051, Basel, Switzerland aut Luka Henryk Research Institute of Organic Agriculture (FiBL), Ackerstrasse 21, 5070, Frick, Switzerland aut Balmer Oliver Research Institute of Organic Agriculture (FiBL), Ackerstrasse 21, 5070, Frick, Switzerland aut BioControl Springer Netherlands 60/2(2015-04-01), 179-187 1386-6141 60:2<179 2015 60 10526 https://doi.org/10.1007/s10526-014-9637-0 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-014-9637-0 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Juric Ivan Research Institute of Organic Agriculture (FiBL), Ackerstrasse 21, 5070, Frick, Switzerland aut NATIONALLICENCE 700 1- Salzburger Walter Zoological Institute, Evolutionary Biology, University of Basel, Vesalgasse 1, 4051, Basel, Switzerland aut NATIONALLICENCE 700 1- Luka Henryk Research Institute of Organic Agriculture (FiBL), Ackerstrasse 21, 5070, Frick, Switzerland aut NATIONALLICENCE 700 1- Balmer Oliver Research Institute of Organic Agriculture (FiBL), Ackerstrasse 21, 5070, Frick, Switzerland aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/2(2015-04-01), 179-187 1386-6141 60:2<179 2015 60 10526