caa a22 4500 606162003 CHVBK 20210128100637.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s10526-014-9643-2 doi (NATIONALLICENCE)springer-10.1007/s10526-014-9643-2 Adding "personality” to biocontrol: characterization and suitability of microsatellites for sibship reconstruction in the aphid parasitoid Diaeretiella rapae [Elektronische Daten] [Isabelle Eisele, Rainer Meyhöfer] Efficiency of natural enemies in pest control in the field frequently shows high degree of variability, making it difficult to propose reliable biocontrol strategies. Due to the small size of many beneficals, underlying mechanisms are difficult to evaluate. In the present study the potential of molecular markers to reconstruct individual foraging decisions of a small parasitoid species unsuitable for direct tracking, i.e. Diaeretiella rapae (M'sIntosh) (Hymenoptera: Braconidae), a primary parasitoid of the cabbage aphid Brevicoryne brassicae (Linnaeus) (Hemiptera: Aphididae), was evaluated. Nine newly designed microsatellite primer pairs were tested for their reliability in parentage analysis. Therefore, several families of D. rapae with four to six daughters and high or low degree of inbreeding were established in the lab. Individuals were genotyped and parentage and sibship analysis was calculated with the software COLONY. The results indicated that 91% of the 85 genotyped offspring from all families have been assigned to the correct family when all nine microsatellite loci have been used and 86% when only six microsatellite loci have been used. Even in families with high degree of inbreeding up to 87% of the offspring could be assigned correctly. In conclusion, the sibship analysis of D. rapae provides reliable results, especially with all nine selected microsatellite loci. Therefore it will be a valuable tool to reconstruct individual foraging decisions and characterize parasitoid "personality” in the field to improve biological control strategies. International Organization for Biological Control (IOBC), 2014 Molecular marker nationallicence Parentage nationallicence Sibling analysis nationallicence COLONY software nationallicence Foraging behavior nationallicence Braconidae nationallicence Eisele Isabelle Section Phytomedicine, Institute of Horticultural Production Systems, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419, Hannover, Germany aut Meyhöfer Rainer Section Phytomedicine, Institute of Horticultural Production Systems, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419, Hannover, Germany aut BioControl Springer Netherlands 60/2(2015-04-01), 189-197 1386-6141 60:2<189 2015 60 10526 https://doi.org/10.1007/s10526-014-9643-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10526-014-9643-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Eisele Isabelle Section Phytomedicine, Institute of Horticultural Production Systems, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419, Hannover, Germany aut NATIONALLICENCE 700 1- Meyhöfer Rainer Section Phytomedicine, Institute of Horticultural Production Systems, Leibniz Universität Hannover, Herrenhäuser Straße 2, 30419, Hannover, Germany aut NATIONALLICENCE 773 0- BioControl Springer Netherlands 60/2(2015-04-01), 189-197 1386-6141 60:2<189 2015 60 10526