caa a22 4500 606162666 CHVBK 20210128100640.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11062-015-9500-7 doi (NATIONALLICENCE)springer-10.1007/s11062-015-9500-7 Suppression of GABAergic Synaptic Transmission by Azidin: Probable Mechanism of a Seizure-Inducing Side Effect [Elektronische Daten] [S. Ivanova, L. Nikolayenko, M. Storozhuk] Azidin (Berenil, Intervet) is a protisticide drug based on diminazene aceturate. It is widely used in veterinary medicine for the treatment and prophylactics of piroplasmosis, babesioses, and teileriosis. Azidin is not used in clinical practice because of the probability of serious side effects, in particular initiation of seizure activity. We examined the effect of Azidin on evoked GABAergic postsynaptic currents (PSTs) in cultured hippocampal neurons using a patch clamp technique. As was found, Azidin in a concentration equivalent to 40 μM diminazene aceturate provides a more than twofold drop in the amplitude of evoked GABAergic PSTs; the effect develops in 3 to 4 min after application. The diminazene concentration in blood serum after its injection into animals can reach 45 μM; this is why the seizure side effect of Azidin can probably be explained mostly by suppression of GABAergic transmission. A combination of Azidin with positive modulators of GABA receptors can be considered an approach allowing one to minimize its side effect. Springer Science+Business Media New York, 2015 diminazene aceturate (Azidin nationallicence Berenil) nationallicence side effects nationallicence seizure activity nationallicence GABAergic synaptic transmission nationallicence Ivanova S. Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine aut Nikolayenko L. Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine aut Storozhuk M. Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine aut Neurophysiology Springer US; http://www.springer-ny.com 47/1(2015-02-01), 77-79 0090-2977 47:1<77 2015 47 11062 https://doi.org/10.1007/s11062-015-9500-7 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11062-015-9500-7 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ivanova S. Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Nikolayenko L. Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 700 1- Storozhuk M. Bogomolets Institute of Physiology, NAS of Ukraine, Kyiv, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Springer US; http://www.springer-ny.com 47/1(2015-02-01), 77-79 0090-2977 47:1<77 2015 47 11062