caa a22 4500 606162747 CHVBK 20210128100640.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s11062-015-9491-4 doi (NATIONALLICENCE)springer-10.1007/s11062-015-9491-4 Effects of Aging and Experimentally Induced Modifications of Signal Pathways on Insulin-Induced Shifts of Glucose Metabolism in the Rat Neocortex [Elektronische Daten] [N. Babenko, V. Kharchenko] We studied insulin-induced intensification of glucose absorption and glycogen formation in the neocortex of young and old rats. In young animals, the corresponding shifts were clearly expressed; in old rats, insulin exerted nearly no stimulatory action on the processes of glucose metabolism in neocortical tissues. It was hypothesized that this is due to the age-related enhancement of the level of ceramides resulting in changes of the lipid spectrum of the cell membranes and also due to suppression of key components of signal pathways of insulin in the brain (such as Akt/protein kinase В, ARF, protein kinase С, and phospholipase D). These events disturb the signal cascade of the hormone and process of formation of the physiological response. An increase in the amount of ceramides in neocortical tissues of young animals after the action of exogenous С2 ceramide or palmitic acid (precursor of sphingolipids) was accompanied by suppression of the intensification of glucose absorption and insulin-stimulated formation of glycogen. Taking into account a significant increase in the amount of ceramides in the neocortex of old animals (this was shown in our earlier study), we believe that age-related accumulation of ceramide is an important reason for the development of insulin resistance of glucose metabolism in the CNS of old organisms. Springer Science+Business Media New York, 2015 neocortex nationallicence aging nationallicence glucose nationallicence glycogen nationallicence insulin nationallicence ceramide nationallicence palmitic acid nationallicence Babenko N. Research Institute of Biology at the Karazin Khar'kiv National University, Khar'kiv, Ukraine aut Kharchenko V. Research Institute of Biology at the Karazin Khar'kiv National University, Khar'kiv, Ukraine aut Neurophysiology Springer US; http://www.springer-ny.com 47/1(2015-02-01), 16-22 0090-2977 47:1<16 2015 47 11062 https://doi.org/10.1007/s11062-015-9491-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11062-015-9491-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Babenko N. Research Institute of Biology at the Karazin Khar'kiv National University, Khar'kiv, Ukraine aut NATIONALLICENCE 700 1- Kharchenko V. Research Institute of Biology at the Karazin Khar'kiv National University, Khar'kiv, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Springer US; http://www.springer-ny.com 47/1(2015-02-01), 16-22 0090-2977 47:1<16 2015 47 11062