caa a22 4500 606162836 CHVBK 20210128100641.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s11062-015-9518-x doi (NATIONALLICENCE)springer-10.1007/s11062-015-9518-x Biophysical Processes in a Urinary Bladder Detrusor Smooth Muscle Cell during Rehabilitation Electrostimulation: a Simulation Study [Elektronische Daten] [А. Kochenov, E. Poddubnaya, I. Makedonsky, S. Korogod] The work was aimed at the search for approaches to solving the problem of biophysically reasonable selection of the parameters of electrical stimulation of smooth muscle cells (SMCs) of the urinary bladder detrusor (UBD). Such stimulation is widely used in the rehabilitation of patients with surgical correction of congenital malformations accompanied by total or partial deficiency of the M2/M3 cholinergic receptors in the UBD. A computer model built on the basis of experimental data on ion channels and pumps of the sarcolemma and mechanisms of regulation of the intracellular calcium concentration ([Ca22+]i), providing both electrogenesis and the contractile function of the cell inherent to the biological prototype, was used. We studied changes in the membrane potential, partial transmembrane currents, and [Ca2+]i, caused by depolarizing current pulses applied with constant frequencies and combined in "packs” or "envelopes” typical of the protocols of rehabilitation stimulation; the stimuli had constant or trapezoid-modulated amplitudes. The examined UBD SMC responded to a single pulse by generation of the action potential (AP) close in its properties to the prototype. Stimulation by both packs and envelopes of identical pulses eventually led to the establishing of equal forced electrical and concentration oscillations with the parameters depending on the duration of interpulse intervals (IPIs). Such oscillations caused by stimulation with 5- and 50-msec-long IPIs, typical of the rehabilitation protocols and comparable with the durations of the absolute and relative refractoriness of the model SMC, significantly differed in the pattern of the regenerative responses (APs) and in the range and mean levels of depolarization shifts of the membrane potential and those of [Ca2+]i, which were greater at high-frequency stimulation. In the case of short IPIs, [Ca2+]i, having no time to return to the basal level, oscillated within a range of values which in other excitable cells are considered to exceed significantly the physiological norm. These data emphasize the necessity to estimate the exact kinetic characteristics of the mechanisms underlying the inflow and extrusion of Ca2+ in the UBD SMC necessary for a biophysically justified choice of the parameters of rehabilitation stimulation that would prevent possible cytotoxic side effects associated with excessively long-lasting high levels of [Ca2+]i. Essential for the observed processes and, therefore, requiring targeted studies, was such a parameter of UBD SMCs as the reversal potential for Ca2+-dependent chloride current (E Cl); this current is activated, in particular, by parasympathetic action on the M2/M3 receptors. When high-frequency oscillations of the membrane potential periodically exceeded the E Cl level, the mentioned current changed its main (depolarizing) direction to the opposite (hyperpolarizing) one. Springer Science+Business Media New York, 2015 mathematical model nationallicence smooth muscle cell (SMC) nationallicence urinary bladder detrusor (UBD) nationallicence electrostimulation nationallicence ion channels nationallicence intracellular calcium nationallicence Kochenov А. Dnipropetrovsk division of the International Center for Molecular Physiology, National Academy of Sciences of Ukraine, Dnipropetrovsk, Ukraine aut Poddubnaya E. Rudnev Dnipropetrovsk Specialized Clinical Medical Center of Mother and Child of the Dnipropetrovsk Regional Council, Dnipropetrovsk, Ukraine aut Makedonsky I. Rudnev Dnipropetrovsk Specialized Clinical Medical Center of Mother and Child of the Dnipropetrovsk Regional Council, Dnipropetrovsk, Ukraine aut Korogod S. Dnipropetrovsk division of the International Center for Molecular Physiology, National Academy of Sciences of Ukraine, Dnipropetrovsk, Ukraine aut Neurophysiology Springer US; http://www.springer-ny.com 47/3(2015-06-01), 174-184 0090-2977 47:3<174 2015 47 11062 https://doi.org/10.1007/s11062-015-9518-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11062-015-9518-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kochenov А. Dnipropetrovsk division of the International Center for Molecular Physiology, National Academy of Sciences of Ukraine, Dnipropetrovsk, Ukraine aut NATIONALLICENCE 700 1- Poddubnaya E. Rudnev Dnipropetrovsk Specialized Clinical Medical Center of Mother and Child of the Dnipropetrovsk Regional Council, Dnipropetrovsk, Ukraine aut NATIONALLICENCE 700 1- Makedonsky I. Rudnev Dnipropetrovsk Specialized Clinical Medical Center of Mother and Child of the Dnipropetrovsk Regional Council, Dnipropetrovsk, Ukraine aut NATIONALLICENCE 700 1- Korogod S. Dnipropetrovsk division of the International Center for Molecular Physiology, National Academy of Sciences of Ukraine, Dnipropetrovsk, Ukraine aut NATIONALLICENCE 773 0- Neurophysiology Springer US; http://www.springer-ny.com 47/3(2015-06-01), 174-184 0090-2977 47:3<174 2015 47 11062