caa a22 4500 606163018 CHVBK 20210128100641.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11062-015-9533-y doi (NATIONALLICENCE)springer-10.1007/s11062-015-9533-y Possible Role of Protein CPG15 in Hippocampal Mossy Fiber Sprouting Under Conditions of Pentylenetetrazole Kindling [Elektronische Daten] [M. Song, F. Tian, J. Dang, W. Huang, J. Guo] We examined changes in expression of the candidate plasticity-related gene 15 (CPG15) in the dentate gyrus (DG) and hippocampal CA3 region in the pentylenetetrazole (PTZ) kindling model and investigated the role of this gene in the phenomenon of mossy fiber sprouting (MFS). Experimental rats were divided into the control and PTZ groups. The epileptic model was created by intraperitoneal PTZ injection, while control rats were injected with saline. At days 3, 7, 14, 28, and 42 after the first PTZ injection, Timm staining was scored in the CA3 hippocampal area, and a product of CPG15 (protein CPG15) was labeled in the DG stratum granulosum and in the CA3 area using immunohistochemistry. The Timm scores in the CA3 region increased gradually from day 3 and were significantly higher than those in the control within the subsequent period. The level of CPG15 protein in the DG and CA3 area decreased gradually until day 14 and returned to the normal level at day 28. The results obtained indicate, for the first time, that CPG15 may be involved in the process of MFS. Understanding the molecular mechanisms underlying this phenomenon may lead to successful therapeutic interventions that limit epileptogenesis. Springer Science+Business Media New York, 2015 protein CPG15 nationallicence mossy fiber sprouting nationallicence temporal lobe epilepsy nationallicence dentate gyrus nationallicence hippocampus nationallicence Song M. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut Tian F. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut Dang J. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut Huang W. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut Guo J. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut Neurophysiology Springer US; http://www.springer-ny.com 47/4(2015-08-01), 271-276 0090-2977 47:4<271 2015 47 11062 https://doi.org/10.1007/s11062-015-9533-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11062-015-9533-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Song M. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut NATIONALLICENCE 700 1- Tian F. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut NATIONALLICENCE 700 1- Dang J. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut NATIONALLICENCE 700 1- Huang W. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut NATIONALLICENCE 700 1- Guo J. Department of Neurology, Xiangya Hospital, Central South University, Changsha, China aut NATIONALLICENCE 773 0- Neurophysiology Springer US; http://www.springer-ny.com 47/4(2015-08-01), 271-276 0090-2977 47:4<271 2015 47 11062