caa a22 4500 606163050 CHVBK 20210128100642.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s11062-015-9535-9 doi (NATIONALLICENCE)springer-10.1007/s11062-015-9535-9 Time Profile of nNOS Expression in the Spinal Dorsal Horn after L5 Spinal Root Transection in Rats [Elektronische Daten] [Z. Bahari, H. Manaheji, L. Dargahi, S. Daniali, M. Norozian, G. Meftahi, M. Sadeghi] Using immunohistochemical analysis, we investigated the time profile of neuronal nitric oxide synthase (nNOS) expression in the lumbar spinal cord up to day 28 after transection of the L5 spinal root. On day 14 after injury, we also evaluated the effect of intrathecal application of 7-nitroindazole (7-NI), a selective nNOS inhibitor (8.15 μg in 5 μl), on thermal hyperalgesia. Our results indicated that nerve transection increased the intensity of nNOS-immunoreactivity in superficial and deep laminae of the dorsal horn within a late stage (days 7 to 28) of the neuropathy model used. Furthermore, 7-NI attenuated nerve injury-evoked thermal hypersensitivity on day 14 but did not reduce it between days 2 and 5 after transection. These data suggest that nNOS overexpression is more involved in the development than in the initiation of thermal hyperalgesia in L5-transected rats. Springer Science+Business Media New York, 2015 neuropathic pain nationallicence spinal dorsal horn nationallicence L5 segment nationallicence nNOS nationallicence 7-nitroindazole nationallicence thermal hyperalgesia nationallicence Bahari Z. Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Manaheji H. Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Dargahi L. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Daniali S. Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Norozian M. Department of Anatomy, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut Meftahi G. Neuroscience Research Center, Baqiyatallah (a.s.), University of Medical Sciences, Tehran, Iran aut Sadeghi M. Department of Physiology, Bushehr University of Medical Sciences, Bushehr, Iran aut Neurophysiology Springer US; http://www.springer-ny.com 47/4(2015-08-01), 287-294 0090-2977 47:4<287 2015 47 11062 https://doi.org/10.1007/s11062-015-9535-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11062-015-9535-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bahari Z. Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Manaheji H. Department of Physiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Dargahi L. Neuroscience Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Daniali S. Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Norozian M. Department of Anatomy, Shahid Beheshti University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Meftahi G. Neuroscience Research Center, Baqiyatallah (a.s.), University of Medical Sciences, Tehran, Iran aut NATIONALLICENCE 700 1- Sadeghi M. Department of Physiology, Bushehr University of Medical Sciences, Bushehr, Iran aut NATIONALLICENCE 773 0- Neurophysiology Springer US; http://www.springer-ny.com 47/4(2015-08-01), 287-294 0090-2977 47:4<287 2015 47 11062