caa a22 4500 606163190 CHVBK 20210128100642.0 cr unu---uuuuu 210128e20150401xx s 000 0 eng 10.1007/s11062-015-9506-1 doi (NATIONALLICENCE)springer-10.1007/s11062-015-9506-1 Caffeine Suppresses GABA Receptor-Mediated Current in Rat Primary Sensory Neurons via Inhibition of Intracellular Phosphodiesterase [Elektronische Daten] [J. Yang, G. Yang, J. Ren, J. Zhao, Sh. Li] In acutely isolated rat primary sensory neurons, the effects of caffeine on GABA receptormediated current (I GABA) were investigated using a whole-cell patch clamp technique. We found that applications of GABA (10-1000 μM) induced inward currents in a concentration-dependent manner; the currents manifested obvious desensitization. Pretreatment with caffeine (0.01-100 μM) suppressed I GABA in a noncompetitive manner; caffeine shifted the concentration-response curve for GABA downwards compared to the control. Theophylline showed a similar and stronger inhibitory effect on I GABA. Isolated application of 1 μM diazepam enhanced I GABA, while pretreatment with 10 μM caffeine and 1 μM diazepam suppressed this current. Intracellular application of the protein kinase A inhibitor H-8 dramatically weakened the inhibitory effect of caffeine on I GABA. Because primary afferent depolarization is related to GABAA receptors, our results suggest that caffeine might antagonize presynaptic inhibitory effects of primary afferents, probably via inhibition of intracellular phosphodiesterase. Springer Science+Business Media New York, 2015 GABA receptor-mesiated current nationallicence primary sensory (DRG) neurons nationallicence whole-cell patch clamp nationallicence caffeine nationallicence phosphodiesterase nationallicence Yang J. Department of Urology, Dalian Friendship Hospital, Dalian, China aut Yang G. Department of Physiology, Dalian, China aut Ren J. Department of Anesthesiology, Jinan Central Hospital, Jinan, China aut Zhao J. Department of Physiology, Dalian, China aut Li Sh Department of Physiology, Dalian, China aut Neurophysiology Springer US; http://www.springer-ny.com 47/2(2015-04-01), 108-114 0090-2977 47:2<108 2015 47 11062 https://doi.org/10.1007/s11062-015-9506-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s11062-015-9506-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yang J. Department of Urology, Dalian Friendship Hospital, Dalian, China aut NATIONALLICENCE 700 1- Yang G. Department of Physiology, Dalian, China aut NATIONALLICENCE 700 1- Ren J. Department of Anesthesiology, Jinan Central Hospital, Jinan, China aut NATIONALLICENCE 700 1- Zhao J. Department of Physiology, Dalian, China aut NATIONALLICENCE 700 1- Li Sh Department of Physiology, Dalian, China aut NATIONALLICENCE 773 0- Neurophysiology Springer US; http://www.springer-ny.com 47/2(2015-04-01), 108-114 0090-2977 47:2<108 2015 47 11062