caa a22 4500 606169660 CHVBK 20210128100713.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s10098-014-0827-3 doi (NATIONALLICENCE)springer-10.1007/s10098-014-0827-3 Application of response surface methodology for the optimization of textile effluent biodecolorization and its toxicity perspectives using plant toxicity, plasmid nicking assays [Elektronische Daten] [Shekhar Jadhav, Amit Chougule, Dhawal Shah, Cristina Pereira, Jyoti Jadhav] Textile effluents are hugely hazardous, and comparatively less research have been focused on their biodecolorization process optimization by single bacterium. Hence, present study was intended toward statistical optimization of textile wastewater biodecolorization by Pseudomonas sp. SUK1 employing response surface methodology. Effects of pH, temperature, and effluent concentrations were analyzed, and an optimal response of 92.20% American Dye Manufacturing Institute (ADMI) value removal was recorded at 7.0 pH, 40°C, and 40% of effluent concentration. This response was maximized to 94.08% ADMI removal from predicted solutions. Actual and predicted values were matching and the process was found suitable for quadratic model. Success of response surface methodology was validated from R 2. Significant interactions of each variable were demonstrated by response surface plots. Bacterium reduced the heavy metals concentration, Biological Oxygen Demand, and Chemical Oxygen Demand values significantly. Degradation of dyes was checked by high performance thin layer chromatography. Effluent treatment caused the nicking of pBR322. Up- /down- regulation of antioxidant enzymes in Allium cepa L. confirmed the severe toxicity of effluent and also suggested that the toxicity was significantly reduced by the bacterial treatment. Springer-Verlag Berlin Heidelberg, 2014 ADMI nationallicence Biodecolorization nationallicence Pseudomonas sp. SUK1 nationallicence Response surface methodology nationallicence Wastewater nationallicence Jadhav Shekhar Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa (ITQB-UNL), Av. da República, 2780-157, Oeiras, Portugal aut Chougule Amit Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, 400614, Navi Mumbai, India aut Shah Dhawal Department of Biotechnology, Shivaji University, 416004, Kolhapur, India aut Pereira Cristina Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa (ITQB-UNL), Av. da República, 2780-157, Oeiras, Portugal aut Jadhav Jyoti Department of Biochemistry, Shivaji University, 416004, Kolhapur, India aut Clean Technologies and Environmental Policy Springer Berlin Heidelberg 17/3(2015-03-01), 709-720 1618-954X 17:3<709 2015 17 10098 https://doi.org/10.1007/s10098-014-0827-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10098-014-0827-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Jadhav Shekhar Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa (ITQB-UNL), Av. da República, 2780-157, Oeiras, Portugal aut NATIONALLICENCE 700 1- Chougule Amit Department of Biotechnology and Bioinformatics, Padmashree Dr. D.Y. Patil University, 400614, Navi Mumbai, India aut NATIONALLICENCE 700 1- Shah Dhawal Department of Biotechnology, Shivaji University, 416004, Kolhapur, India aut NATIONALLICENCE 700 1- Pereira Cristina Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa (ITQB-UNL), Av. da República, 2780-157, Oeiras, Portugal aut NATIONALLICENCE 700 1- Jadhav Jyoti Department of Biochemistry, Shivaji University, 416004, Kolhapur, India aut NATIONALLICENCE 773 0- Clean Technologies and Environmental Policy Springer Berlin Heidelberg 17/3(2015-03-01), 709-720 1618-954X 17:3<709 2015 17 10098