caa a22 4500 60617060X CHVBK 20210128100718.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10098-014-0783-y doi (NATIONALLICENCE)springer-10.1007/s10098-014-0783-y Biodegradation of azo dyes in industrial effluent: an eco-friendly way toward green technology [Elektronische Daten] [J. Kanagaraj, T. Senthilvelan, R. Panda] Laccase enzyme obtained from Micrococcus luteus was exploited for biodegradation of azo dyes. The enzyme was characterized for optimum growth conditions at pH 7.0, temperature at 37°C and incubation duration of 72h. The molecular weight of the enzyme was found to be 63kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis. Application of enzyme on the two dyes namely C.I. Acid Black 52 and C.I. Acid Blue 113 resulted in biodegradation rate of 92.2 and 94.5%, respectively. UV-Vis spectra analysis indicated the absence of peaks in the visible region of the experimental samples confirmed the complete biodegradation of dye samples. Further, Fourier transform-infrared spectroscopy results showed the transformation of N=N into N2 or NH3 and then into biomass. Intermediate products of the biodegraded dye sample were analyzed by mass-electrospray ionization spectra. The reduction in BOD and COD values were found to be 93.2, 92.5 and 92.0, 88.7%, respectively, for the above dyes. Springer-Verlag Berlin Heidelberg, 2014 Azo dyes nationallicence Laccase nationallicence Biodegradation nationallicence Modeling nationallicence Mass and FT-IR spectra analysis nationallicence Pollution reduction nationallicence Kanagaraj J. Leather Processing Division, CSIR- Central Leather Research Institute, Adyar, 600020, Chennai, India aut Senthilvelan T. Leather Processing Division, CSIR- Central Leather Research Institute, Adyar, 600020, Chennai, India aut Panda R. Leather Processing Division, CSIR- Central Leather Research Institute, Adyar, 600020, Chennai, India aut Clean Technologies and Environmental Policy Springer Berlin Heidelberg 17/2(2015-02-01), 331-341 1618-954X 17:2<331 2015 17 10098 https://doi.org/10.1007/s10098-014-0783-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10098-014-0783-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kanagaraj J. Leather Processing Division, CSIR- Central Leather Research Institute, Adyar, 600020, Chennai, India aut NATIONALLICENCE 700 1- Senthilvelan T. Leather Processing Division, CSIR- Central Leather Research Institute, Adyar, 600020, Chennai, India aut NATIONALLICENCE 700 1- Panda R. Leather Processing Division, CSIR- Central Leather Research Institute, Adyar, 600020, Chennai, India aut NATIONALLICENCE 773 0- Clean Technologies and Environmental Policy Springer Berlin Heidelberg 17/2(2015-02-01), 331-341 1618-954X 17:2<331 2015 17 10098