caa a22 4500 606170626 CHVBK 20210128100718.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s10098-014-0791-y doi (NATIONALLICENCE)springer-10.1007/s10098-014-0791-y Eco-friendly enzymatic dehairing of skins and hides by C. brefeldianus protease [Elektronische Daten] [Harish Khandelwal, Snehal More, K. Kalal, R. Laxman] Alkaline protease from Conidiobolus brefeldianus was efficient in unhairing various types of skins and hides. The crude protease preparation was active toward keratin-azure, elastin-orcin, azocasein, and azocoll, but did not show true collagenase activity. In addition, the crude enzyme exhibited other enzyme activities such as chondroitinase, laminarase, and chitinase. Complete hair removal of skin/hide by the protease achieved in 16-18h. The dehaired pelt showed smooth and white appearance due to hair removal along with epidermal layer. In addition, the grain was clean and without damage in enzymatically dehaired pelts. The microscopic observation of the cross-section of dehaired goat skin and cow hide showed absence of epidermis, hair shaft with empty follicles. Enzymatic dehairing resulted in complete and uniform fiber opening in the dermis and corium region. Physical properties viz. tensile strength, elongation, and tear strength of dyed crust of enzymatically and conventionally dehaired pelts were comparable. Results were also validated on large scale with goat skins and cow hides. Springer-Verlag Berlin Heidelberg, 2014 Conidiobolus brefeldianus nationallicence Alkaline protease nationallicence Enzymatic dehairing nationallicence Sheep skins nationallicence Cow hides nationallicence Dehaired pelts nationallicence Khandelwal Harish Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India aut More Snehal Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India aut Kalal K. Division of Organic Chemistry, National Chemical Laboratory, 411 008, Pune, India aut Laxman R. Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India aut Clean Technologies and Environmental Policy Springer Berlin Heidelberg 17/2(2015-02-01), 393-405 1618-954X 17:2<393 2015 17 10098 https://doi.org/10.1007/s10098-014-0791-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s10098-014-0791-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Khandelwal Harish Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India aut NATIONALLICENCE 700 1- More Snehal Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India aut NATIONALLICENCE 700 1- Kalal K. Division of Organic Chemistry, National Chemical Laboratory, 411 008, Pune, India aut NATIONALLICENCE 700 1- Laxman R. Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India aut NATIONALLICENCE 773 0- Clean Technologies and Environmental Policy Springer Berlin Heidelberg 17/2(2015-02-01), 393-405 1618-954X 17:2<393 2015 17 10098