caa a22 4500 606174788 CHVBK 20210128100739.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00018-015-1889-z doi (NATIONALLICENCE)springer-10.1007/s00018-015-1889-z O -GlcNAc modification is essential for the regulation of autophagy in Drosophila melanogaster [Elektronische Daten] [Sujin Park, Yangsin Lee, Jin Pak, Hanbyeol Kim, Hyeonjin Choi, Jae-woo Kim, Jürgen Roth, Jin Cho] O-GlcNAcylation is a dynamic post-translational modification that takes place on ser/thr residues of nucleocytoplasmic proteins. O-GlcNAcylation regulates almost all cellular events as a nutrient sensor, a transcriptional and translational regulator, and a disease-related factor. Although the role of O-GlcNAcylation in insulin signaling and metabolism are well established, the relationship between O-GlcNAcylation and autophagy is largely unknown. Here, we manipulated O-GlcNAcylation in Drosophila and found that it regulates autophagy through Akt/dFOXO signaling. We demonstrate that O-GlcNAcylation and the levels of O-GlcNAc transferase (OGT) are increased during starvation. Furthermore, Atg proteins and autolysosomes are increased in OGT-reduced flies without fasting. Atg proteins and autophagosomes are reduced in OGT-overexpressing flies. Our results suggest that not only autophagy gene expression but also autophagic structures are regulated by OGT through Akt and dFOXO. These data imply that O-GlcNAcylation is important in modulating autophagy as well as insulin signaling in Drosophila. Springer Basel, 2015 O -GlcNAc modification nationallicence OGT nationallicence Autophagy nationallicence Drosophila melanogaster nationallicence dFOXO nationallicence Park Sujin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut Lee Yangsin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut Pak Jin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut Kim Hanbyeol Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut Choi Hyeonjin Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, Korea aut Kim Jae-woo Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, Korea aut Roth Jürgen Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut Cho Jin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut Cellular and Molecular Life Sciences Springer Basel 72/16(2015-08-01), 3173-3183 1420-682X 72:16<3173 2015 72 18 https://doi.org/10.1007/s00018-015-1889-z text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1889-z text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Park Sujin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut NATIONALLICENCE 700 1- Lee Yangsin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut NATIONALLICENCE 700 1- Pak Jin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut NATIONALLICENCE 700 1- Kim Hanbyeol Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut NATIONALLICENCE 700 1- Choi Hyeonjin Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Kim Jae-woo Department of Biochemistry and Molecular Biology, Integrated Genomic Research Center for Metabolic Regulation, Institute of Genetic Science, Yonsei University College of Medicine, Seoul, Korea aut NATIONALLICENCE 700 1- Roth Jürgen Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut NATIONALLICENCE 700 1- Cho Jin Department of Integrated OMICS for Biomedical Science, Graduate School, Yonsei University, 50 Yonsei-ro, Seodaemun-gu, 120-749, Seoul, South Korea aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/16(2015-08-01), 3173-3183 1420-682X 72:16<3173 2015 72 18