caa a22 4500 606174826 CHVBK 20210128100739.0 cr unu---uuuuu 210128e20150801xx s 000 0 eng 10.1007/s00018-015-1914-2 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1914-2 Control of polarized assembly of actin filaments in cell motility [Elektronische Daten] [Marie-France Carlier, Julien Pernier, Pierre Montaville, Shashank Shekhar, Sonja Kühn] Actin cytoskeleton remodeling, which drives changes in cell shape and motility, is orchestrated by a coordinated control of polarized assembly of actin filaments. Signal responsive, membrane-bound protein machineries initiate and regulate polarized growth of actin filaments by mediating transient links with their barbed ends, which elongate from polymerizable actin monomers. The barbed end of an actin filament thus stands out as a hotspot of regulation of filament assembly. It is the target of both soluble and membrane-bound agonists as well as antagonists of filament assembly. Here, we review the molecular mechanisms by which various regulators of actin dynamics bind, synergize or compete at filament barbed ends. Two proteins can compete for the barbed end via a mutually exclusive binding scheme. Alternatively, two regulators acting individually at barbed ends may be bound together transiently to terminal actin subunits at barbed ends, leading to the displacement of one by the other. The kinetics of these reactions is a key in understanding how filament length and membrane-filament linkage are controlled. It is also essential for understanding how force is produced to shape membranes by mechano-sensitive, processive barbed end tracking machineries like formins and by WASP-Arp2/3 branched filament arrays. A combination of biochemical and biophysical approaches, including bulk solution assembly measurements using pyrenyl-actin fluorescence, single filament dynamics, single molecule fluorescence imaging and reconstituted self-organized filament assemblies, have provided mechanistic insight into the role of actin polymerization in motile processes. The Author(s), 2015 Actin filament barbed end nationallicence Treadmilling nationallicence Processivity nationallicence Formins nationallicence WH2 domains nationallicence WASP family proteins nationallicence Arp2/3 complex nationallicence Filament branching nationallicence Capping proteins nationallicence Profilin nationallicence Cell protrusion nationallicence Carlier Marie-France Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut Pernier Julien Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut Montaville Pierre Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut Shekhar Shashank Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut Kühn Sonja Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut Cellular and Molecular Life Sciences Springer Basel 72/16(2015-08-01), 3051-3067 1420-682X 72:16<3051 2015 72 18 https://doi.org/10.1007/s00018-015-1914-2 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1914-2 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Carlier Marie-France Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut NATIONALLICENCE 700 1- Pernier Julien Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut NATIONALLICENCE 700 1- Montaville Pierre Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut NATIONALLICENCE 700 1- Shekhar Shashank Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut NATIONALLICENCE 700 1- Kühn Sonja Institut de Biologie Intégrative de la Cellule, CNRS, 91198, Gif-sur Yvette, France aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/16(2015-08-01), 3051-3067 1420-682X 72:16<3051 2015 72 18