caa a22 4500 606174893 CHVBK 20210128100740.0 cr unu---uuuuu 210128e20151001xx s 000 0 eng 10.1007/s00018-015-1952-9 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1952-9 Mechanisms for exporting large-sized cargoes from the endoplasmic reticulum [Elektronische Daten] [Kota Saito, Toshiaki Katada] Cargo proteins exported from the endoplasmic reticulum to the Golgi apparatus are typically transported in coat protein complex II (COPII)-coated vesicles of 60-90nm diameter. Several cargo molecules including collagens and chylomicrons form structures that are too large to be accommodated by these vesicles, but their secretion still requires COPII proteins. Here, we first review recent progress on large cargo secretions derived especially from animal models and human diseases, which indicate the importance of COPII proteins. We then discuss the recent isolation of specialized factors that modulate the process of COPII-dependent cargo formation to facilitate the exit of large-sized cargoes from the endoplasmic reticulum. Based on these findings, we propose a model that describes the importance of the GTPase cycle for secretion of oversized cargoes. Next, we summarize reports that describe the structures of COPII proteins and how these results provide insight into the mechanism of assembly of the large cargo carriers. Finally, we discuss what issues remain to be solved in the future. The Author(s), 2015 COPII nationallicence Collagen nationallicence Chylomicron nationallicence TANGO1 nationallicence cTAGE5 nationallicence CMRD : Chylomicron retension disease nationallicence COPII : Coat protein complex II nationallicence CLSD : Cranio-lenticulo-sutural dysplasia nationallicence ECM : Extracellular matrix nationallicence ER : Endoplasmic reticulum nationallicence GEF : Guanine-nucleotide exchange factor nationallicence MIA : Melanoma inhibitory activity nationallicence MSS : Marinesco-Sjogren syndrome nationallicence NPC : Nuclear pore complex nationallicence TANGO : Transport ANd Golgi Organization nationallicence TRAPP : TRAnsport Protein Particle nationallicence Saito Kota Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut Katada Toshiaki Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut Cellular and Molecular Life Sciences Springer Basel 72/19(2015-10-01), 3709-3720 1420-682X 72:19<3709 2015 72 18 https://doi.org/10.1007/s00018-015-1952-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1952-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito Kota Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut NATIONALLICENCE 700 1- Katada Toshiaki Department of Physiological Chemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033, Tokyo, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/19(2015-10-01), 3709-3720 1420-682X 72:19<3709 2015 72 18