caa a22 4500 606175016 CHVBK 20210128100741.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00018-014-1735-8 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1735-8 Prion strains are differentially released through the exosomal pathway [Elektronische Daten] [Zaira Arellano-Anaya, Alvina Huor, Pascal Leblanc, Sylvain Lehmann, Monique Provansal, Graça Raposo, Olivier Andréoletti, Didier Vilette] Cell-to-cell transfer of prions is a crucial step in the spreading of prion infection through infected tissue. At the cellular level, several distinct pathways including direct cell-cell contacts and release of various types of infectious extracellular vesicles have been described that may potentially lead to infection of naïve cells. The relative contribution of these pathways and whether they may vary depending on the prion strain and/or on the infected cell type are not yet known. In this study we used a single cell type (RK13) infected with three different prion strains. We showed that in each case, most of the extracellular prions resulted from active cell secretion through the exosomal pathway. Further, quantitative analysis of secreted infectivity indicated that the proportion of prions eventually secreted was dramatically dependent on the prion strain. Our data also highlight that infectious exosomes secreted from cultured cells might represent a biologically pertinent material for spiking experiments. Also discussed is the appealing possibility that abnormal PrP from different prion strains may differentially interact with the cellular machinery to promote secretion. Springer Basel, 2014 Prion release nationallicence Cell models nationallicence Exosome nationallicence Extracellular vesicle nationallicence Prion spiking nationallicence TSE agent nationallicence Arellano-Anaya Zaira INRA, UMR 1225, IHAP, 31076, Toulouse, France aut Huor Alvina INRA, UMR 1225, IHAP, 31076, Toulouse, France aut Leblanc Pascal Laboratoire de Biologie Moléculaire de la Cellule (LBMC), Equipe Différenciation Neuromusculaire, Ecole Normale Supérieure-Lyon, CNRS, UMR 5239, 46 allée d'Italie, 69364, Lyon Cedex 07, France aut Lehmann Sylvain Institut de Médecine Régénératrice et de Biothérapie (I.M.R.B.), Physiopathologie, Diagnostic et Thérapie Cellulaire des Affections Neurodégénératives, Institut National de la Santé et de la Recherche Médicale Université Montpellier 1 U1040 Centre Hospitalo-Universitaire de Montpellier, Université Montpellier 1, Montpellier, France aut Provansal Monique Institut de Médecine Régénératrice et de Biothérapie (I.M.R.B.), Physiopathologie, Diagnostic et Thérapie Cellulaire des Affections Neurodégénératives, Institut National de la Santé et de la Recherche Médicale Université Montpellier 1 U1040 Centre Hospitalo-Universitaire de Montpellier, Université Montpellier 1, Montpellier, France aut Raposo Graça Institut Curie, UMR 144, CNRS, Structure and Membrane Compartments, Cell and Tissue Imaging Facility (PICT-IBiSA), 26 rue d'Ulm, 75248, Paris Cedex 05, France aut Andréoletti Olivier INRA, UMR 1225, IHAP, 31076, Toulouse, France aut Vilette Didier INRA, UMR 1225, IHAP, 31076, Toulouse, France aut Cellular and Molecular Life Sciences Springer Basel 72/6(2015-03-01), 1185-1196 1420-682X 72:6<1185 2015 72 18 https://doi.org/10.1007/s00018-014-1735-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1735-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Arellano-Anaya Zaira INRA, UMR 1225, IHAP, 31076, Toulouse, France aut NATIONALLICENCE 700 1- Huor Alvina INRA, UMR 1225, IHAP, 31076, Toulouse, France aut NATIONALLICENCE 700 1- Leblanc Pascal Laboratoire de Biologie Moléculaire de la Cellule (LBMC), Equipe Différenciation Neuromusculaire, Ecole Normale Supérieure-Lyon, CNRS, UMR 5239, 46 allée d'Italie, 69364, Lyon Cedex 07, France aut NATIONALLICENCE 700 1- Lehmann Sylvain Institut de Médecine Régénératrice et de Biothérapie (I.M.R.B.), Physiopathologie, Diagnostic et Thérapie Cellulaire des Affections Neurodégénératives, Institut National de la Santé et de la Recherche Médicale Université Montpellier 1 U1040 Centre Hospitalo-Universitaire de Montpellier, Université Montpellier 1, Montpellier, France aut NATIONALLICENCE 700 1- Provansal Monique Institut de Médecine Régénératrice et de Biothérapie (I.M.R.B.), Physiopathologie, Diagnostic et Thérapie Cellulaire des Affections Neurodégénératives, Institut National de la Santé et de la Recherche Médicale Université Montpellier 1 U1040 Centre Hospitalo-Universitaire de Montpellier, Université Montpellier 1, Montpellier, France aut NATIONALLICENCE 700 1- Raposo Graça Institut Curie, UMR 144, CNRS, Structure and Membrane Compartments, Cell and Tissue Imaging Facility (PICT-IBiSA), 26 rue d'Ulm, 75248, Paris Cedex 05, France aut NATIONALLICENCE 700 1- Andréoletti Olivier INRA, UMR 1225, IHAP, 31076, Toulouse, France aut NATIONALLICENCE 700 1- Vilette Didier INRA, UMR 1225, IHAP, 31076, Toulouse, France aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/6(2015-03-01), 1185-1196 1420-682X 72:6<1185 2015 72 18