caa a22 4500 606175334 CHVBK 20210128100742.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00018-014-1829-3 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1829-3 Autophagy and cell reprogramming [Elektronische Daten] [Shuo Wang, Pengyan Xia, Markus Rehm, Zusen Fan] Autophagy is an evolutionarily conserved process that degrades cytoplasmic components, thus contributing to cell survival and tissue homeostasis. Recent studies have demonstrated that autophagy maintains stem cells in relatively undifferentiated states (stemness) and also contributes to differentiation processes. Autophagy likewise plays a crucial role in somatic cell reprogramming, a finely regulated process that resets differentiated cells to a pluripotent state and that requires comprehensive alterations in transcriptional activities and epigenetic signatures. Autophagy assists in manifesting the functional consequences that arise from these alterations by modifying cellular protein expression profiles. The role of autophagy appears to be particularly relevant for early phases of cell reprogramming during the generation of induced pluripotent stems cells (iPSCs). In this review, we provide an overview of the core molecular machinery that constitutes the autophagic degradation system, describe the roles of autophagy in maintenance, self-renewal, and differentiation of stem cells, and discuss the autophagic process and its regulation during cell reprogramming. Springer Basel, 2015 Autophagy nationallicence Stem cells nationallicence Self-renewal nationallicence Differentiation nationallicence Cell reprogramming nationallicence Ambra1 : Activating molecule in Beclin1-regulated autophagy 1 nationallicence AMPK : AMP-activated protein kinase nationallicence Atg : Autophagy-related proteins nationallicence Beclin1 : Coiled-coil, myosin-like BCL2-interacting protein nationallicence BafA1 : Bafilomycin A1 nationallicence CSC : Cancer stem cell nationallicence CHD : Chromodomain, helicase, DNA binding nationallicence EMT : Epithelial-mesenchymal transition nationallicence ESC : Embryonic stem cell nationallicence FGF : Fibroblast growth factor nationallicence GBM : Glioblastoma nationallicence GAP : GTPase-activating protein nationallicence HSC : Hematopoietic stem cell nationallicence iPSC : Induced pluripotent stem cell nationallicence LC3 : Microtubule-associated protein light chain 3 nationallicence LIF : Leukemia inhibitory factor nationallicence MBD3 : Methyl-CpG binding domain protein 3 nationallicence mTOR : Mammalian target of rapamycin nationallicence NuRD : Nucleosome remodeling and deacetylase nationallicence NSC : Neural stem cell nationallicence PE : Phosphatidylethanolamine nationallicence Rheb : Ras homologue enriched in brain nationallicence ROS : Reactive oxygen species nationallicence Sox2 : SRY (sex determining region Y)-box 2 nationallicence SVZ : Subventricular zone nationallicence TSC1/2 : Tuberous sclerosis1/2 nationallicence ULK : Unc-51-like kinase nationallicence WASH : Wiskott-Aldrich syndrome protein and SCAR homologue nationallicence Wang Shuo CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China aut Xia Pengyan CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China aut Rehm Markus Department of Physiology and Medical Physics, Centre for Systems Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland aut Fan Zusen CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China aut Cellular and Molecular Life Sciences Springer Basel 72/9(2015-05-01), 1699-1713 1420-682X 72:9<1699 2015 72 18 https://doi.org/10.1007/s00018-014-1829-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1829-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Wang Shuo CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Xia Pengyan CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 700 1- Rehm Markus Department of Physiology and Medical Physics, Centre for Systems Medicine, Royal College of Surgeons in Ireland, Dublin, Ireland aut NATIONALLICENCE 700 1- Fan Zusen CAS Key Laboratory of Infection and Immunity, Institute of Biophysics, Chinese Academy of Sciences, 100101, Beijing, China aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/9(2015-05-01), 1699-1713 1420-682X 72:9<1699 2015 72 18