caa a22 4500 606175598 CHVBK 20210128100743.0 cr unu---uuuuu 210128e20150601xx s 000 0 eng 10.1007/s00018-014-1816-8 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1816-8 Metabolic insights from zebrafish genetics, physiology, and chemical biology [Elektronische Daten] [Amnon Schlegel, Philipp Gut] Metabolic diseases—atherosclerotic cardiovascular disease, type 2 diabetes mellitus, obesity, and non-alcoholic fatty liver disease--have reached pandemic proportions. Across gene, cell, organ, organism, and social-environmental scales, fundamental discoveries of the derangements that occur in these diseases are required to develop effective new treatments. Here we will review genetic, physiological, pathological and chemical biological discoveries in the emerging zebrafish model for studying metabolism and metabolic diseases. We present a synthesis of recent studies using forward and reverse genetic tools to make new contributions to our understanding of lipid trafficking, diabetes pathogenesis and complications, and to β-cell biology. The technical and physiological advantages and the pharmacological potential of this organism for discovery and validation of metabolic disease targets are stressed by our summary of recent findings. We conclude by arguing that metabolic research using zebrafish will benefit from adoption of conventional blood and tissue metabolite measurements, employment of modern imaging techniques, and development of more rigorous metabolic flux methods. The Author(s), 2015 Atherosclerosis nationallicence Diabetes mellitus nationallicence Metabolism nationallicence Zebrafish nationallicence β-cell nationallicence Schlegel Amnon University of Utah Molecular Medicine Program, School of Medicine, University of Utah, 15 North 2030 East, Room 3240B, 84112, Salt Lake City, UT, USA aut Gut Philipp Nestlé Institute of Health Sciences, EPFL Innovation Park, Bâtiment H, 1015, Lausanne, Switzerland aut Cellular and Molecular Life Sciences Springer Basel 72/12(2015-06-01), 2249-2260 1420-682X 72:12<2249 2015 72 18 https://doi.org/10.1007/s00018-014-1816-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1816-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Schlegel Amnon University of Utah Molecular Medicine Program, School of Medicine, University of Utah, 15 North 2030 East, Room 3240B, 84112, Salt Lake City, UT, USA aut NATIONALLICENCE 700 1- Gut Philipp Nestlé Institute of Health Sciences, EPFL Innovation Park, Bâtiment H, 1015, Lausanne, Switzerland aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/12(2015-06-01), 2249-2260 1420-682X 72:12<2249 2015 72 18