caa a22 4500 606175717 CHVBK 20210128100744.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00018-015-1998-8 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1998-8 Complexins: small but capable [Elektronische Daten] [Ralf Mohrmann, Madhurima Dhara, Dieter Bruns] Despite intensive research, it is still unclear how an immediate and profound acceleration of exocytosis is triggered by appropriate Ca2+-stimuli in presynaptic terminals. This is due to the fact that the molecular mechanisms of "docking” and "priming” reactions, which set up secretory vesicles to fuse at millisecond time scale, are extremely hard to study. Yet, driven by a fruitful combination of in vitro and in vivo analyses, our mechanistic understanding of Ca2+-triggered vesicle fusion has certainly advanced in the past few years. In this review, we aim to highlight recent progress and emerging views on the molecular mechanisms, by which constitutively forming SNAREpins are organized in functional, tightly regulated units for synchronized release. In particular, we will focus on the role of the small regulatory factor complexin whose function in Ca2+-dependent exocytosis has been controversially discussed for more than a decade. Special emphasis will also be laid on the functional relationship of complexin and synaptotagmin, as both proteins possibly act as allies and/or antagonists to govern SNARE-mediated exocytosis. The Author(s), 2015 Complexin nationallicence Synaptotagmin nationallicence SNARE regulators nationallicence Membrane fusion nationallicence Ca2+ triggered exocytosis nationallicence AH : Accessory α-helix nationallicence EM : Electron micrograph nationallicence [Ca]i : Intracellular calcium nationallicence CH : Central helix nationallicence cplx : Complexin nationallicence CT : C-Terminus nationallicence hGH : Human growth hormone nationallicence PC12 : Pheochromocytoma cell line nationallicence SER : Serine nationallicence SNAREs : N-Ethylmaleimide-sensitive factor (NSF) attachment protein receptors nationallicence SNAP25 : Synaptosomal-associated protein, 25kDa nationallicence sybII : Synaptobrevin II nationallicence stxIa : Syntaxin Ia nationallicence syt : Synaptotagmin nationallicence NT : N-Terminus nationallicence NMJ : Neuromuscular junction nationallicence Mohrmann Ralf Zentrum für Human- und Molekularbiologie, University of Saarland, CIPMM, 66421, Homburg/Saar, Germany aut Dhara Madhurima Medical Faculty, Department of Physiology, University of Saarland, CIPMM, 66421, Homburg/Saar, Germany aut Bruns Dieter Medical Faculty, Department of Physiology, University of Saarland, CIPMM, 66421, Homburg/Saar, Germany aut Cellular and Molecular Life Sciences Springer Basel 72/22(2015-11-01), 4221-4235 1420-682X 72:22<4221 2015 72 18 https://doi.org/10.1007/s00018-015-1998-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1998-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mohrmann Ralf Zentrum für Human- und Molekularbiologie, University of Saarland, CIPMM, 66421, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- Dhara Madhurima Medical Faculty, Department of Physiology, University of Saarland, CIPMM, 66421, Homburg/Saar, Germany aut NATIONALLICENCE 700 1- Bruns Dieter Medical Faculty, Department of Physiology, University of Saarland, CIPMM, 66421, Homburg/Saar, Germany aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/22(2015-11-01), 4221-4235 1420-682X 72:22<4221 2015 72 18