caa a22 4500 60617575X CHVBK 20210128100744.0 cr unu---uuuuu 210128e20151101xx s 000 0 eng 10.1007/s00018-015-2002-3 doi (NATIONALLICENCE)springer-10.1007/s00018-015-2002-3 Fast-folding proteins under stress [Elektronische Daten] [Kapil Dave, Martin Gruebele] Proteins are subject to a variety of stresses in biological organisms, including pressure and temperature, which are the easiest stresses to simulate by molecular dynamics. We discuss the effect of pressure and thermal stress on very-fast-folding model proteins, whose in vitro folding can be fully simulated on computers and compared with experiments. We then discuss experiments that can be used to subject proteins to low- and high-temperature unfolding, as well as low- and high-pressure unfolding. Pressure and temperature are prototypical perturbations that illustrate how close many proteins are to instability, a property that cells can exploit to control protein function. We conclude by reviewing some recent in-cell experiments, and progress being made in simulating and measuring protein stability and function inside live cells. Springer Basel, 2015 Phase diagram nationallicence WW domain nationallicence Temperature jump nationallicence NTL9 nationallicence Pressure jump nationallicence Proton NMR nationallicence Fluorescence nationallicence Cell cycle nationallicence Molecular dynamics nationallicence Dave Kapil Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut Gruebele Martin Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut Cellular and Molecular Life Sciences Springer Basel 72/22(2015-11-01), 4273-4285 1420-682X 72:22<4273 2015 72 18 https://doi.org/10.1007/s00018-015-2002-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-2002-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dave Kapil Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut NATIONALLICENCE 700 1- Gruebele Martin Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/22(2015-11-01), 4273-4285 1420-682X 72:22<4273 2015 72 18