caa a22 4500 606175776 CHVBK 20210128100744.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00018-015-1899-x doi (NATIONALLICENCE)springer-10.1007/s00018-015-1899-x Kir3 channels undergo arrestin-dependant internalization following delta opioid receptor activation [Elektronische Daten] [Karim Nagi, Iness Charfi, Graciela Pineyro] Kir3 channels control excitability in the nervous system and the heart. Their surface expression is strictly regulated, but mechanisms responsible for channel removal from the membrane remain incompletely understood. Using transfected cells, we show that Kir3.1/3.2 channels and delta opioid receptors (DORs) associate in a complex which persists during receptor activation, behaving as a scaffold that allows beta-arrestin (βarr) to interact with both signaling partners. This organization favored co-internalization of DORs and Kir3 channels in a βarr-dependent manner via a clathrin/dynamin-mediated endocytic path. Taken together, these findings identify a new way of modulating Kir3 channel availability at the membrane and assign a putatively novel role for βarrs in regulating canonical effectors for G protein-coupled receptors. Springer Basel, 2015 Kir channels nationallicence Trafficking nationallicence Signaling complexes nationallicence Arrestin nationallicence Endocytosis nationallicence Opioid nationallicence Nagi Karim Sainte-Justine Hospital Research Center, 3175 Cote Ste-Catherine, H3T 1C5, Montreal, QC, Canada aut Charfi Iness Sainte-Justine Hospital Research Center, 3175 Cote Ste-Catherine, H3T 1C5, Montreal, QC, Canada aut Pineyro Graciela Sainte-Justine Hospital Research Center, 3175 Cote Ste-Catherine, H3T 1C5, Montreal, QC, Canada aut Cellular and Molecular Life Sciences Springer Basel 72/18(2015-09-01), 3543-3557 1420-682X 72:18<3543 2015 72 18 https://doi.org/10.1007/s00018-015-1899-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1899-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nagi Karim Sainte-Justine Hospital Research Center, 3175 Cote Ste-Catherine, H3T 1C5, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Charfi Iness Sainte-Justine Hospital Research Center, 3175 Cote Ste-Catherine, H3T 1C5, Montreal, QC, Canada aut NATIONALLICENCE 700 1- Pineyro Graciela Sainte-Justine Hospital Research Center, 3175 Cote Ste-Catherine, H3T 1C5, Montreal, QC, Canada aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/18(2015-09-01), 3543-3557 1420-682X 72:18<3543 2015 72 18