caa a22 4500 606175784 CHVBK 20210128100744.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00018-015-1905-3 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1905-3 Maternal PCBP1 determines the normal timing of pronucleus formation in mouse eggs [Elektronische Daten] [Zhonghua Shi, Chun Zhao, Ye Yang, Hui Teng, Ying Guo, Minyue Ma, Xuejiang Guo, Zuomin Zhou, Ran Huo, Qi Zhou] In mammals, pronucleus formation, a landmark event for egg activation and fertilization, is critical for embryonic development. However, the mechanisms underlying pronucleus formation remain unclear. Increasing evidence has shown that the transition from a mature egg to a developing embryo and the early steps of development are driven by the control of maternal cytoplasmic factors. Herein, a two-dimensional-electrophoresis-based proteomic approach was used in metaphase II and parthenogenetically activated mouse eggs to search for maternal proteins involved in egg activation, one of which was poly(rC)-binding protein 1 (PCBP1). Phosphoprotein staining indicated that PCBP1 displayed dephosphorylation in parthenogenetically activated egg, which possibly boosts its ability to bind to mRNAs. We identified 75 mRNAs expressed in mouse eggs that contained the characteristic PCBP1-binding CU-rich sequence in the 3′-UTR. Among them, we focused on H2a.x mRNA, as it was closely related to pronucleus formation in Xenopus oocytes. Further studies suggested that PCBP1 could bind to H2a.x mRNA and enhance its stability, thus promoting mouse pronucleus formation during parthenogenetic activation of murine eggs, while the inhibition of PCBP1 evidently retarded pronucleus formation. In summary, these data propose that PCBP1 may serve as a novel maternal factor that is required for determining the normal timing of pronucleus formation. Springer Basel, 2015 hnRNP E1 nationallicence mRNA stabilization nationallicence Pronuclear development nationallicence Microinjection nationallicence Shi Zhonghua State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Zhao Chun State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Yang Ye State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Teng Hui State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Guo Ying State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Ma Minyue State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Guo Xuejiang State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Zhou Zuomin State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Huo Ran State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut Zhou Qi State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut Cellular and Molecular Life Sciences Springer Basel 72/18(2015-09-01), 3575-3586 1420-682X 72:18<3575 2015 72 18 https://doi.org/10.1007/s00018-015-1905-3 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1905-3 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shi Zhonghua State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhao Chun State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Yang Ye State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Teng Hui State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Guo Ying State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Ma Minyue State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Guo Xuejiang State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Zuomin State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Huo Ran State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, 210029, Nanjing, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Qi State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, People's Republic of China aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/18(2015-09-01), 3575-3586 1420-682X 72:18<3575 2015 72 18