caa a22 4500 606176063 CHVBK 20210128100745.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00018-015-1842-1 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1842-1 Exogenous enzymes upgrade transgenesis and genetic engineering of farm animals [Elektronische Daten] [Pablo Bosch, Diego Forcato, Fabrisio Alustiza, Ana Alessio, Alejandro Fili, María Olmos Nicotra, Ana Liaudat, Nancy Rodríguez, Thirumala Talluri, Wilfried Kues] Transgenic farm animals are attractive alternative mammalian models to rodents for the study of developmental, genetic, reproductive and disease-related biological questions, as well for the production of recombinant proteins, or the assessment of xenotransplants for human patients. Until recently, the ability to generate transgenic farm animals relied on methods of passive transgenesis. In recent years, significant improvements have been made to introduce and apply active techniques of transgenesis and genetic engineering in these species. These new approaches dramatically enhance the ease and speed with which livestock species can be genetically modified, and allow to performing precise genetic modifications. This paper provides a synopsis of enzyme-mediated genetic engineering in livestock species covering the early attempts employing naturally occurring DNA-modifying proteins to recent approaches working with tailored enzymatic systems. Springer Basel, 2015 Active transgenesis nationallicence Livestock nationallicence Binary transposon system nationallicence Designer nuclease nationallicence Recombinase nationallicence Integrase nationallicence Synthetic biology nationallicence Cas9 : CRISPR-associated protein 9 nationallicence CPI : Cytoplasmic injection nationallicence Ct : Chromatin transfer nationallicence CRISPR : Clustered regularly interspaced short palindromic repeats nationallicence DSB : Double-strand break nationallicence GOI : Gene of interest nationallicence HR : Homologous recombination nationallicence HDR : Homology-directed repair nationallicence ICSI : Intracytoplasmic sperm injection nationallicence ICSI-Tr : Intracytoplasmic sperm injection-mediated transgenesis nationallicence iPS : Induced pluripotent stem (cell) nationallicence I-SceI : Homing endonuclease nationallicence ITR : Inverted terminal repeat nationallicence KO : Knockout nationallicence NHEJ : Non-homologous end joining nationallicence PB : piggyBac transposon system nationallicence PNI : Pronuclear injection nationallicence RE : Restriction enzyme nationallicence RecA : Recombinase A nationallicence REMI : Restriction enzyme-mediated integration nationallicence RMCE : Recombinase-mediated cassette exchange nationallicence RMDI : Recombinase-mediated DNA insertion nationallicence SB : Sleeping Beauty transposon system nationallicence SCNT : Somatic cell nuclear transfer nationallicence sgRNA : Single-guide RNA nationallicence SMGT : Sperm-mediated gene transfer nationallicence SV40 : Simian virus 40 nationallicence TALEN : Transcription activator-like element nuclease nationallicence Tol2 : Tol2 transposon system nationallicence ZFN : Zinc finger nuclease nationallicence Bosch Pablo Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Forcato Diego Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Alustiza Fabrisio Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Alessio Ana Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Fili Alejandro Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Olmos Nicotra María Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Liaudat Ana Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Rodríguez Nancy Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut Talluri Thirumala Friedrich-Loeffler-Institute, Institute of Farm Animal Genetics, Biotechnology, 31535, Neustadt, Germany aut Kues Wilfried Friedrich-Loeffler-Institute, Institute of Farm Animal Genetics, Biotechnology, 31535, Neustadt, Germany aut Cellular and Molecular Life Sciences Springer Basel 72/10(2015-05-01), 1907-1929 1420-682X 72:10<1907 2015 72 18 https://doi.org/10.1007/s00018-015-1842-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1842-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bosch Pablo Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Forcato Diego Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Alustiza Fabrisio Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Alessio Ana Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Fili Alejandro Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Olmos Nicotra María Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Liaudat Ana Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Rodríguez Nancy Departamento de Biología Molecular, Facultad de Ciencias Exactas, Fco-Qcas y Naturales, Universidad Nacional de Río Cuarto, Río Cuarto, Córdoba, Republic of Argentina aut NATIONALLICENCE 700 1- Talluri Thirumala Friedrich-Loeffler-Institute, Institute of Farm Animal Genetics, Biotechnology, 31535, Neustadt, Germany aut NATIONALLICENCE 700 1- Kues Wilfried Friedrich-Loeffler-Institute, Institute of Farm Animal Genetics, Biotechnology, 31535, Neustadt, Germany aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/10(2015-05-01), 1907-1929 1420-682X 72:10<1907 2015 72 18