caa a22 4500 60617611X CHVBK 20210128100746.0 cr unu---uuuuu 210128e20150501xx s 000 0 eng 10.1007/s00018-014-1795-9 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1795-9 EGF is required for cardiac differentiation of P19CL6 cells through interaction with GATA-4 in a time- and dose-dependent manner [Elektronische Daten] [Cai-Xia Ma, Yang-Liu Song, Liyun Xiao, Li-Xiang Xue, Wen-Juan Li, Brigitte Laforest, Hiba Komati, Wei-Ping Wang, Zhu-Qing Jia, Chun-Yan Zhou, Yunzeng Zou, Mona Nemer, Shan-Feng Zhang, Xiaowen Bai, Huijian Wu, Ming-Xi Zang] The regulation of cardiac differentiation is critical for maintaining normal cardiac development and function. The precise mechanisms whereby cardiac differentiation is regulated remain uncertain. Here, we have identified a GATA-4 target, EGF, which is essential for cardiogenesis and regulates cardiac differentiation in a dose- and time-dependent manner. Moreover, EGF demonstrates functional interaction with GATA-4 in inducing the cardiac differentiation of P19CL6 cells in a time- and dose-dependent manner. Biochemically, GATA-4 forms a complex with STAT3 to bind to the EGF promoter in response to EGF stimulation and cooperatively activate the EGF promoter. Functionally, the cooperation during EGF activation results in the subsequent activation of cyclin D1 expression, which partly accounts for the lack of additional induction of cardiac differentiation by the GATA-4/STAT3 complex. Thus, we propose a model in which the regulatory cascade of cardiac differentiation involves GATA-4, EGF, and cyclin D1. Springer Basel, 2014 Cardiogenesis nationallicence Sarcomeric myosin heavy chain nationallicence Pluripotent mouse embryonal carcinoma cells nationallicence Signaling transduction nationallicence Gene regulation nationallicence Ma Cai-Xia Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut Song Yang-Liu Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut Xiao Liyun School of Life Science and Biotechnology, Dalian University of Technology, 2 Ling Gong Road, 116024, Dalian, China aut Xue Li-Xiang Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut Li Wen-Juan Department of Pediatric Cardiology, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, 200092, Shanghai, China aut Laforest Brigitte Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, K1N 6N5, Ottawa, ON, Canada aut Komati Hiba Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, K1N 6N5, Ottawa, ON, Canada aut Wang Wei-Ping Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut Jia Zhu-Qing Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut Zhou Chun-Yan Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut Zou Yunzeng Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, and Institutes of Biomedical Sciences, Fudan University, 200032, Shanghai, China aut Nemer Mona Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, K1N 6N5, Ottawa, ON, Canada aut Zhang Shan-Feng Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut Bai Xiaowen Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, 53226, Milwaukee, WI, USA aut Wu Huijian School of Life Science and Biotechnology, Dalian University of Technology, 2 Ling Gong Road, 116024, Dalian, China aut Zang Ming-Xi Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut Cellular and Molecular Life Sciences Springer Basel 72/10(2015-05-01), 2005-2022 1420-682X 72:10<2005 2015 72 18 https://doi.org/10.1007/s00018-014-1795-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1795-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ma Cai-Xia Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Song Yang-Liu Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Xiao Liyun School of Life Science and Biotechnology, Dalian University of Technology, 2 Ling Gong Road, 116024, Dalian, China aut NATIONALLICENCE 700 1- Xue Li-Xiang Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Li Wen-Juan Department of Pediatric Cardiology, Xinhua Hospital, Affiliated to Shanghai Jiao Tong University School of Medicine, 1665 Kongjiang Road, 200092, Shanghai, China aut NATIONALLICENCE 700 1- Laforest Brigitte Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, K1N 6N5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Komati Hiba Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, K1N 6N5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Wang Wei-Ping Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Jia Zhu-Qing Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Zhou Chun-Yan Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Peking University, 100191, Beijing, China aut NATIONALLICENCE 700 1- Zou Yunzeng Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, and Institutes of Biomedical Sciences, Fudan University, 200032, Shanghai, China aut NATIONALLICENCE 700 1- Nemer Mona Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, K1N 6N5, Ottawa, ON, Canada aut NATIONALLICENCE 700 1- Zhang Shan-Feng Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 700 1- Bai Xiaowen Department of Anesthesiology, Medical College of Wisconsin, 8701 Watertown Plank Road, 53226, Milwaukee, WI, USA aut NATIONALLICENCE 700 1- Wu Huijian School of Life Science and Biotechnology, Dalian University of Technology, 2 Ling Gong Road, 116024, Dalian, China aut NATIONALLICENCE 700 1- Zang Ming-Xi Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Zhengzhou University, Ke Xue Da Dao 100, 450001, Zhengzhou, Henan, China aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/10(2015-05-01), 2005-2022 1420-682X 72:10<2005 2015 72 18