caa a22 4500 606176381 CHVBK 20210128100747.0 cr unu---uuuuu 210128e20150901xx s 000 0 eng 10.1007/s00018-015-1898-y doi (NATIONALLICENCE)springer-10.1007/s00018-015-1898-y Direct visualization of vaults within intact cells by electron cryo-tomography [Elektronische Daten] [Cora Woodward, Luiza Mendonça, Grant Jensen] The vault complex is the largest cellular ribonucleoprotein complex ever characterized and is present across diverse Eukarya. Despite significant information regarding the structure, composition and evolutionary conservation of the vault, little is know about the complex's actual biological function. To determine if intracellular vaults are morphologically similar to previously studied purified and recombinant vaults, we have used electron cryo-tomography to characterize the vault complexes found in the thin edges of primary human cells growing in tissue culture. Our studies confirm that intracellular vaults are similar in overall size and shape to purified and recombinant vaults previously analyzed. Results from subtomogram averaging indicate that densities within the vault lumen are not ordered, but randomly distributed. We also observe that vaults located in the extreme periphery of the cytoplasm predominately associate with granule-like structures and actin. Our ultrastructure studies augment existing biochemical, structural and genetic information on the vault, and provide important intracellular context for the ongoing efforts to understand the biological function of the native cytoplasmic vault. Springer Basel, 2015 Vault nationallicence Ribonucleoprotein complex nationallicence Granules nationallicence RNA nationallicence Electron cryo-tomography nationallicence Woodward Cora Division of Biology, California Institute of Technology, 1200 E California Blvd, 91125, Pasadena, CA, USA aut Mendonça Luiza Division of Biology, California Institute of Technology, 1200 E California Blvd, 91125, Pasadena, CA, USA aut Jensen Grant Division of Biology, California Institute of Technology, 1200 E California Blvd, 91125, Pasadena, CA, USA aut Cellular and Molecular Life Sciences Springer Basel 72/17(2015-09-01), 3401-3409 1420-682X 72:17<3401 2015 72 18 https://doi.org/10.1007/s00018-015-1898-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1898-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Woodward Cora Division of Biology, California Institute of Technology, 1200 E California Blvd, 91125, Pasadena, CA, USA aut NATIONALLICENCE 700 1- Mendonça Luiza Division of Biology, California Institute of Technology, 1200 E California Blvd, 91125, Pasadena, CA, USA aut NATIONALLICENCE 700 1- Jensen Grant Division of Biology, California Institute of Technology, 1200 E California Blvd, 91125, Pasadena, CA, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/17(2015-09-01), 3401-3409 1420-682X 72:17<3401 2015 72 18