caa a22 4500 606176667 CHVBK 20210128100748.0 cr unu---uuuuu 210128e20150201xx s 000 0 eng 10.1007/s00018-014-1770-5 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1770-5 Bacterial chemoreceptors and chemoeffectors [Elektronische Daten] [Shuangyu Bi, Luhua Lai] Bacteria use chemotaxis signaling pathways to sense environmental changes. Escherichia coli chemotaxis system represents an ideal model that illustrates fundamental principles of biological signaling processes. Chemoreceptors are crucial signaling proteins that mediate taxis toward a wide range of chemoeffectors. Recently, in deep study of the biochemical and structural features of chemoreceptors, the organization of higher-order clusters in native cells, and the signal transduction mechanisms related to the on-off signal output provides us with general insights to understand how chemotaxis performs high sensitivity, precise adaptation, signal amplification, and wide dynamic range. Along with the increasing knowledge, bacterial chemoreceptors can be engineered to sense novel chemoeffectors, which has extensive applications in therapeutics and industry. Here we mainly review recent advances in the E. coli chemotaxis system involving structure and organization of chemoreceptors, discovery, design, and characterization of chemoeffectors, and signal recognition and transduction mechanisms. Possible strategies for changing the specificity of bacterial chemoreceptors to sense novel chemoeffectors are also discussed. Springer Basel, 2014 Chemotactic response nationallicence Receptor nationallicence Ligand nationallicence Molecular mechanism nationallicence Receptor design nationallicence BPs : Periplasmic substrate-binding proteins nationallicence CW : Clockwise nationallicence CCW : Counter-clockwise nationallicence TM2 : The second transmembrane helix nationallicence HAMP : Histidine kinase, adenylyl cyclase, methyl-accepting chemotaxis proteins and phosphatase nationallicence MH : Methylation helix nationallicence NMR : Nuclear magnetic resonance nationallicence MBP : Maltose binding protein nationallicence CHDCA : cis-1,2-Cyclohexane-dicarboxylic acid nationallicence cis -PDA : cis-(2R, 3S)-2,3-Piperidine dicarboxylic acid nationallicence ITC : Isothermal titration calorimetry nationallicence FRET : Fluorescence resonance energy transfer nationallicence EPR : Electron paramagnetic resonance nationallicence PDS : Pulsed dipolar electron spin resonance spectroscopy nationallicence E. coli : Escherichia coli nationallicence A. fulgidus : Archaeoglobus fulgidus nationallicence P. aeruginosa : Pseudomonas aeruginosa nationallicence T. maritima : Thermotoga maritima nationallicence P. putida : Pseudomonas putida nationallicence N. pharaonis : Natronomonas pharaonis nationallicence B. subtilis : Bacillus subtilis nationallicence M. xanthus : Myxococcus xanthus nationallicence Bi Shuangyu Center for Quantitative Biology, Peking University, 100871, Beijing, China aut Lai Luhua Center for Quantitative Biology, Peking University, 100871, Beijing, China aut Cellular and Molecular Life Sciences Springer Basel 72/4(2015-02-01), 691-708 1420-682X 72:4<691 2015 72 18 https://doi.org/10.1007/s00018-014-1770-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1770-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bi Shuangyu Center for Quantitative Biology, Peking University, 100871, Beijing, China aut NATIONALLICENCE 700 1- Lai Luhua Center for Quantitative Biology, Peking University, 100871, Beijing, China aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/4(2015-02-01), 691-708 1420-682X 72:4<691 2015 72 18