caa a22 4500 60617687X CHVBK 20210128100749.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00018-014-1824-8 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1824-8 Laccase engineering by rational and evolutionary design [Elektronische Daten] [Isabel Pardo, Susana Camarero] Laccases are considered as green catalysts of great biotechnological potential. This has attracted a great interest in designing laccases a la carte with enhanced stabilities or activities tailored to specific conditions for different fields of application. Over 20years, numerous efforts have been taken to engineer these multicopper oxidases and to understand their reaction mechanisms by site-directed mutagenesis, and more recently, using computational calculations and directed evolution tools. In this work, we review the most relevant contributions made in the field of laccase engineering, from the comprehensive study of their structure-function relationships to the tailoring of outstanding biocatalysts. The Author(s), 2015 Laccase nationallicence Site-directed mutagenesis nationallicence Random mutagenesis nationallicence Directed evolution nationallicence Computational simulation nationallicence Protein engineering nationallicence Pardo Isabel Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain aut Camarero Susana Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain aut Cellular and Molecular Life Sciences Springer Basel 72/5(2015-03-01), 897-910 1420-682X 72:5<897 2015 72 18 https://doi.org/10.1007/s00018-014-1824-8 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1824-8 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Pardo Isabel Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain aut NATIONALLICENCE 700 1- Camarero Susana Centro de Investigaciones Biológicas, CSIC, Ramiro de Maeztu 9, 28040, Madrid, Spain aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/5(2015-03-01), 897-910 1420-682X 72:5<897 2015 72 18