caa a22 4500 60617690X CHVBK 20210128100749.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00018-014-1726-9 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1726-9 CHD3 facilitates vRNP nuclear export by interacting with NES1 of influenza A virus NS2 [Elektronische Daten] [Yong Hu, Xiaokun Liu, Anding Zhang, Hongbo Zhou, Ziduo Liu, Huanchun Chen, Meilin Jin] NS2 from influenza A virus mediates Crm1-dependent vRNP nuclear export through interaction with Crm1. However, even though the nuclear export signal 1 (NES1) of NS2 does not play a requisite role in NS2-Crm1 interaction, there is no doubt that NES1 is crucial for vRNP nuclear export. While the mechanism of the NES1 is still unclear, it is speculated that certain host partners might mediate the NES1 function through their interaction with NES1. In the present study, chromodomain-helicase-DNA-binding protein 3 (CHD3) was identified as a novel host nuclear protein for locating NS2 and Crm1 on dense chromatin for NS2 and Crm1-dependent vRNP nuclear export. CHD3 was confirmed to interact with NES1 in NS2, and a disruption to this interaction by mutation in NES1 significantly delayed viral vRNPs export and viral propagation. Further, the knockdown of CHD3 would affect the propagation of the wild-type virus but not the mutant with the weakened NS2-CHD3 interaction. Therefore, this study demonstrates that NES1 is required for maximal binding of NS2 to CHD3, and that the NS2-CHD3 interaction on the dense chromatin contributed to the NS2-mediated vRNP nuclear export. The Author(s), 2014 Influenza A virus nationallicence CHD3 nationallicence Dense chromatin nationallicence VRNPs export nationallicence CHD3 : Chromodomain-helicase-DNA-binding protein 3 nationallicence cCHD3 : C-terminus of human CHD3 nationallicence GST : Glutathione S transferase nationallicence IAV : Influenza A virus nationallicence IFA : Immunofluorescence assay nationallicence IP : Immunoprecipitation nationallicence M2H : Mammalian two-hybrid nationallicence NEP : Nuclear export protein nationallicence NES : Nuclear export signal nationallicence Pfu : Plaque-forming units nationallicence siRNAs : Small interfering RNAs nationallicence Y2H : Yeast two-hybrid nationallicence WT : Wild-type nationallicence Hu Yong State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Liu Xiaokun State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Zhang Anding State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Zhou Hongbo State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Liu Ziduo College of Life Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Chen Huanchun State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Jin Meilin State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut Cellular and Molecular Life Sciences Springer Basel 72/5(2015-03-01), 971-982 1420-682X 72:5<971 2015 72 18 https://doi.org/10.1007/s00018-014-1726-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1726-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hu Yong State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Liu Xiaokun State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Zhang Anding State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Zhou Hongbo State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Liu Ziduo College of Life Science and Technology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Chen Huanchun State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 700 1- Jin Meilin State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, 430070, Wuhan, People's Republic of China aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/5(2015-03-01), 971-982 1420-682X 72:5<971 2015 72 18