caa a22 4500 606176918 CHVBK 20210128100749.0 cr unu---uuuuu 210128e20150301xx s 000 0 eng 10.1007/s00018-014-1732-y doi (NATIONALLICENCE)springer-10.1007/s00018-014-1732-y The cellular origin of laminin determines its role in blood pressure regulation [Elektronische Daten] [Yao Yao, Erin Norris, Sidney Strickland] Laminin of different cellular sources has distinct functions. In addition to vascular smooth muscle cells (SMCs), aorta also contains a small population of nestin+ cells, whose function remains unknown. This study investigates the role of SMC- and nestin+ cell-derived laminin in blood pressure (BP) regulation and SMC contractibility. Using mice with laminin deficiency in SMCs (SKO) or nestin+ cells (NKO), we examined laminin-dependent changes in BP. Contractile protein expression was reduced in SKO but not NKO mice, consistent with their, respectively, low and normal baseline BP measurements. At the ultrastructural level, SKO SMCs maintained the contractile phenotype with reduced elasticity, whereas NKO SMCs switched to the synthetic phenotype and showed degeneration. Additionally, angiotensin II (Ang II) significantly increased BP in SKO but not NKO mice. It also enhanced contractile proteins to the same levels and induced SMC degeneration in both knockout mice. These data suggest that SMC laminin regulates BP via modulating contractile protein expression, whereas nestin+ cell-derived laminin contributes to SMC phenotypic switch. Springer Basel, 2014 Laminin nationallicence Nestin nationallicence Smooth muscle cells nationallicence Aorta nationallicence BP : Blood pressure nationallicence Ctr : Control nationallicence F/F : Laminin γ1flox/flox nationallicence SKO : Laminin γ1flox/flox: SM22α-cre+ nationallicence NKO : Laminin γ1flox/flox: nestin-cre+ nationallicence SMC : Smooth muscle cells nationallicence SMA : Smooth muscle actin-α nationallicence Ang II : Angiotensin II nationallicence ATR1 : Angiotensin II receptor type-I nationallicence ATR2 : Angiotensin II receptor type-II nationallicence ECM : Extracellular matrix nationallicence EL : Elastin laminae nationallicence Yao Yao Laboratory of Neurobiology and Genetics, The Rockefeller University, 1230 York Ave, Box 169, 10065, New York, NY, USA aut Norris Erin Laboratory of Neurobiology and Genetics, The Rockefeller University, 1230 York Ave, Box 169, 10065, New York, NY, USA aut Strickland Sidney Laboratory of Neurobiology and Genetics, The Rockefeller University, 1230 York Ave, Box 169, 10065, New York, NY, USA aut Cellular and Molecular Life Sciences Springer Basel 72/5(2015-03-01), 999-1008 1420-682X 72:5<999 2015 72 18 https://doi.org/10.1007/s00018-014-1732-y text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1732-y text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yao Yao Laboratory of Neurobiology and Genetics, The Rockefeller University, 1230 York Ave, Box 169, 10065, New York, NY, USA aut NATIONALLICENCE 700 1- Norris Erin Laboratory of Neurobiology and Genetics, The Rockefeller University, 1230 York Ave, Box 169, 10065, New York, NY, USA aut NATIONALLICENCE 700 1- Strickland Sidney Laboratory of Neurobiology and Genetics, The Rockefeller University, 1230 York Ave, Box 169, 10065, New York, NY, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/5(2015-03-01), 999-1008 1420-682X 72:5<999 2015 72 18