caa a22 4500 606176942 CHVBK 20210128100749.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00018-015-2020-1 doi (NATIONALLICENCE)springer-10.1007/s00018-015-2020-1 RETRACTED ARTICLE: In vitro derivation of mammalian germ cells from stem cells and their potential therapeutic application [Elektronische Daten] [Shigeo Saito, Ying-Chu Lin, Yoshinobu Murayama, Yukio Nakamura, Richard Eckner, Heiner Niemann, Kazunari Yokoyama] Pluripotent stem cells (PSCs) are a unique type of cells because they exhibit the characteristics of self-renewal and pluripotency. PSCs may be induced to differentiate into any cell type, even male and female germ cells, suggesting their potential as novel cell-based therapeutic treatment for infertility problems. Spermatogenesis is an intricate biological process that starts from self-renewal of spermatogonial stem cells (SSCs) and leads to differentiated haploid spermatozoa. Errors at any stage in spermatogenesis may result in male infertility. During the past decade, much progress has been made in the derivation of male germ cells from various types of progenitor stem cells. Currently, there are two main approaches for the derivation of functional germ cells from PSCs, either the induction of in vitro differentiation to produce haploid cell products, or combination of in vitro differentiation and in vivo transplantation. The production of mature and fertile spermatozoa from stem cells might provide an unlimited source of autologous gametes for treatment of male infertility. Here, we discuss the current state of the art regarding the differentiation potential of SSCs, embryonic stem cells, and induced pluripotent stem cells to produce functional male germ cells. We also discuss the possible use of livestock-derived PSCs as a novel option for animal reproduction and infertility treatment. The Author(s), 2015 Animal reproduction nationallicence Embryonic stem cells nationallicence Gametes nationallicence Germ cells nationallicence Primordial germ cells nationallicence Spermatogonial stem cells nationallicence Sterility nationallicence Therapeutic use nationallicence AR : Androgen receptor nationallicence bFGF : Basic fibroblast growth factor nationallicence BMP : Bone morphogenic protein nationallicence DNMT3 : DNA methyltransferase 3 nationallicence DNMT3L : DNA methyltransferase 3-like nationallicence E : Embryonic nationallicence EBs : Embryoid bodies nationallicence ESCs : Embryonic stem cells nationallicence EpiSCs : Epiblast stem cells nationallicence FACS : Fluorescence-activated cell sorting nationallicence GM : Genetically modified nationallicence GDNF : Glial cell line-derived neurotrophic factor nationallicence hESCs : Human embryonic stem cells nationallicence hiESCs : Human induced embryonic stem cells nationallicence hiPSCs : Human induced pluripotent stem cells nationallicence ICM : Inner cell mass nationallicence ICSI : Intracytoplasmic sperm injection nationallicence iPSCs : Induced pluripotent stem cells nationallicence LIF : Leukemia inhibitory factor nationallicence mESCs : Mouse embryonic stem cells nationallicence MEHP : Mono-(2-ethylhexyl) phthalate nationallicence miRNA : Micro-RNA nationallicence Mvh : Mouse vase homologue nationallicence PGCs : Primordial germ cells nationallicence PSCs : Pluripotent stem cells nationallicence SSCs : Spermatogonial stem cells nationallicence SSEA-1 : Stage specific antigen-1 nationallicence SSEA-4 : Stage specific antigen-4 nationallicence TET1 : Ten-eleven translocation methylcytosine dioxygenase nationallicence TP2 : Transition protein 2 nationallicence Saito Shigeo Saito Laboratory of Cell Technology, 329-1571, Yaita, Tochigi, Japan aut Lin Ying-Chu School of Dentistry, College of Dental Medicine, Kaoshiung Medical University, 100 Shin-Chuan 1st Road, 807, Kaohsiung, Taiwan aut Murayama Yoshinobu College of Engineering, Nihon University, 963-8642, Koriyama, Fukushima, Japan aut Nakamura Yukio Cell Engineering Division, RIKEN BioResource Center, 3050074, Tsukuba, Japan aut Eckner Richard Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Rutgers, The State University of New Jersey, 07101, Newark, NJ, USA aut Niemann Heiner Institute of Farm Animal Genetics, Friedrich-Löffler-Institut, Mariensee, 31535, Neustadt, Germany aut Yokoyama Kazunari Graduate Institute of Medicine, Center of Stem Cell Research, Center of Environmental Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Rd, San Ming District, 807, Kaohsiung, Taiwan aut Cellular and Molecular Life Sciences Springer International Publishing 72/23(2015-12-01), 4545-4560 1420-682X 72:23<4545 2015 72 18 https://doi.org/10.1007/s00018-015-2020-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-2020-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito Shigeo Saito Laboratory of Cell Technology, 329-1571, Yaita, Tochigi, Japan aut NATIONALLICENCE 700 1- Lin Ying-Chu School of Dentistry, College of Dental Medicine, Kaoshiung Medical University, 100 Shin-Chuan 1st Road, 807, Kaohsiung, Taiwan aut NATIONALLICENCE 700 1- Murayama Yoshinobu College of Engineering, Nihon University, 963-8642, Koriyama, Fukushima, Japan aut NATIONALLICENCE 700 1- Nakamura Yukio Cell Engineering Division, RIKEN BioResource Center, 3050074, Tsukuba, Japan aut NATIONALLICENCE 700 1- Eckner Richard Department of Biochemistry and Molecular Biology, Rutgers New Jersey Medical School, Rutgers, The State University of New Jersey, 07101, Newark, NJ, USA aut NATIONALLICENCE 700 1- Niemann Heiner Institute of Farm Animal Genetics, Friedrich-Löffler-Institut, Mariensee, 31535, Neustadt, Germany aut NATIONALLICENCE 700 1- Yokoyama Kazunari Graduate Institute of Medicine, Center of Stem Cell Research, Center of Environmental Medicine, Kaohsiung Medical University, 100 Shih-Chuan 1st Rd, San Ming District, 807, Kaohsiung, Taiwan aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer International Publishing 72/23(2015-12-01), 4545-4560 1420-682X 72:23<4545 2015 72 18