caa a22 4500 606176993 CHVBK 20210128100750.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00018-015-1957-4 doi (NATIONALLICENCE)springer-10.1007/s00018-015-1957-4 Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses [Elektronische Daten] [Laura Hildebrand, Petra Seemann, Andreas Kurtz, Jochen Hecht, Jörg Contzen, Manfred Gossen, Harald Stachelscheid] Human induced pluripotent stem cells (hiPSC) differentiate into multiple cell types. Selective cell targeting is often needed for analyzing gene function by overexpressing proteins in a distinct population of hiPSC-derived cell types and for monitoring cell fate in response to stimuli. However, to date, this has not been possible, as commonly used viruses enter the hiPSC via ubiquitously expressed receptors. Here, we report for the first time the application of a heterologous avian receptor, the tumor virus receptor A (TVA), to selectively transduce TVA+ cells in a mixed cell population. Expression of the TVA surface receptor via genetic engineering renders cells susceptible for infection by avian leucosis virus (ALV). We generated hiPSC lines with this stably integrated, ectopic TVA receptor gene that expressed the receptor while retaining pluripotency. The undifferentiated hiPSCTVA+ as well as their differentiating progeny could be infected by recombinant ALV (so-called RCAS virus) with high efficiency. Due to incomplete receptor blocking, even sequential infection of differentiating or undifferentiated TVA+ cells was possible. In conclusion, the TVA/RCAS system provides an efficient and gentle gene transfer system for hiPSC and extends our possibilities for selective cell targeting and lineage tracing studies. Springer Basel, 2015 Induced pluripotent stem cells nationallicence TVA nationallicence ALV nationallicence Selective cell targeting nationallicence Lineage tracing nationallicence Hildebrand Laura Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut Seemann Petra Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut Kurtz Andreas Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut Hecht Jochen Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut Contzen Jörg Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany aut Gossen Manfred Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany aut Stachelscheid Harald Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut Cellular and Molecular Life Sciences Springer International Publishing 72/23(2015-12-01), 4671-4680 1420-682X 72:23<4671 2015 72 18 https://doi.org/10.1007/s00018-015-1957-4 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-1957-4 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hildebrand Laura Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut NATIONALLICENCE 700 1- Seemann Petra Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut NATIONALLICENCE 700 1- Kurtz Andreas Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut NATIONALLICENCE 700 1- Hecht Jochen Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut NATIONALLICENCE 700 1- Contzen Jörg Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany aut NATIONALLICENCE 700 1- Gossen Manfred Berlin-Brandenburg Center for Regenerative Therapies (BCRT), Berlin, Germany aut NATIONALLICENCE 700 1- Stachelscheid Harald Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer International Publishing 72/23(2015-12-01), 4671-4680 1420-682X 72:23<4671 2015 72 18