caa a22 4500 606177000 CHVBK 20210128100750.0 cr unu---uuuuu 210128e20151201xx s 000 0 eng 10.1007/s00018-015-2016-x doi (NATIONALLICENCE)springer-10.1007/s00018-015-2016-x Latarcins: versatile spider venom peptides [Elektronische Daten] [Peter Dubovskii, Alexander Vassilevski, Sergey Kozlov, Alexey Feofanov, Eugene Grishin, Roman Efremov] Arthropod venoms feature the presence of cytolytic peptides believed to act synergetically with neurotoxins to paralyze prey or deter aggressors. Many of them are linear, i.e., lack disulfide bonds. When isolated from the venom, or obtained by other means, these peptides exhibit common properties. They are cationic; being mostly disordered in aqueous solution, assume amphiphilic α-helical structure in contact with lipid membranes; and exhibit general cytotoxicity, including antifungal, antimicrobial, hemolytic, and anticancer activities. To suit the pharmacological needs, the activity spectrum of these peptides should be modified by rational engineering. As an example, we provide a detailed review on latarcins (Ltc), linear cytolytic peptides from Lachesana tarabaevi spider venom. Diverse experimental and computational techniques were used to investigate the spatial structure of Ltc in membrane-mimicking environments and their effects on model lipid bilayers. The antibacterial activity of Ltc was studied against a panel of Gram-negative and Gram-positive bacteria. In addition, the action of Ltc on erythrocytes and cancer cells was investigated in detail with confocal laser scanning microscopy. In the present review, we give a critical account of the progress in the research of Ltc. We explore the relationship between Ltc structure and their biological activity and derive molecular characteristics, which can be used for optimization of other linear peptides. Current applications of Ltc and prospective use of similar membrane-active peptides are outlined. Springer Basel, 2015 Biologically active compounds nationallicence Mechanism of action nationallicence Cytolytic toxin nationallicence Correlation analysis nationallicence Antimicrobial peptide nationallicence Structure-function relationship nationallicence AMP : Antimicrobial peptides nationallicence CBF : Carboxyfluorescein nationallicence CD : Circular dichroism nationallicence CL : Cardiolipin nationallicence CSA : Chemical shift anisotropy nationallicence CT : Cobra venom cytotoxins (cardiotoxins) nationallicence DOPC : Dioleoylphosphatidylcholine nationallicence DOPE : Dioleoylphosphatidylethanolamine nationallicence DOPG : Dioleoylphosphatidylglycerol nationallicence DPhPC : Diphytanoylphosphatidylcholine nationallicence EC50 : Effective concentration producing a half-maximal effect nationallicence EST : Expressed sequence tags nationallicence GAG : Glycosaminoglycan nationallicence iPQM : Inverted PQM nationallicence LP : Linear peptides nationallicence LPS : Lipopolysaccharide nationallicence Ltc : Latarcins nationallicence MC : Monte Carlo method nationallicence MD : Molecular dynamics nationallicence MHP : Molecular hydrophobicity potential nationallicence MIC : Minimal inhibitory concentration nationallicence PE : Phosphatidylethanolamine nationallicence PG : Phosphatidylglycerol nationallicence P/L : Peptide to lipid molar ratio nationallicence PQM : Processing quadruplet motif nationallicence PS : Phosphatidylserine nationallicence PTG : Peptidoglycan nationallicence RPE : Repetitive polypeptide elements nationallicence SDS : Sodium dodecyl sulfate nationallicence TFE : Trifluoroethanol nationallicence TI : Therapeutic index nationallicence n.d. : Not determined nationallicence Dubovskii Peter Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Vassilevski Alexander Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Kozlov Sergey Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Feofanov Alexey Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Grishin Eugene Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Efremov Roman Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut Cellular and Molecular Life Sciences Springer International Publishing 72/23(2015-12-01), 4501-4522 1420-682X 72:23<4501 2015 72 18 https://doi.org/10.1007/s00018-015-2016-x text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-015-2016-x text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Dubovskii Peter Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Vassilevski Alexander Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Kozlov Sergey Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Feofanov Alexey Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Grishin Eugene Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 700 1- Efremov Roman Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya, 117997, Moscow, Russia aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer International Publishing 72/23(2015-12-01), 4501-4522 1420-682X 72:23<4501 2015 72 18