caa a22 4500 606177086 CHVBK 20210128100750.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00018-014-1738-5 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1738-5 Microscale screening systems for 3D cellular microenvironments: platforms, advances, and challenges [Elektronische Daten] [Sara Montanez-Sauri, David Beebe, Kyung Sung] The increasing interest in studying cells using more in vivo-like three-dimensional (3D) microenvironments has created a need for advanced 3D screening platforms with enhanced functionalities and increased throughput. 3D screening platforms that better mimic in vivo microenvironments with enhanced throughput would provide more in-depth understanding of the complexity and heterogeneity of microenvironments. The platforms would also better predict the toxicity and efficacy of potential drugs in physiologically relevant conditions. Traditional 3D culture models (e.g., spinner flasks, gyratory rotation devices, non-adhesive surfaces, polymers) were developed to create 3D multicellular structures. However, these traditional systems require large volumes of reagents and cells, and are not compatible with high-throughput screening (HTS) systems. Microscale technology offers the miniaturization of 3D cultures and allows efficient screening of various conditions. This review will discuss the development, most influential works, and current advantages and challenges of microscale culture systems for screening cells in 3D microenvironments. Springer Basel, 2014 Microarray nationallicence Extracellular matrix nationallicence Microwells nationallicence Technology nationallicence Collagen nationallicence Hydrogels nationallicence Microfluidics nationallicence 2D : Two-dimensional nationallicence 3D : Three-dimensional nationallicence APA : Alginate-poly-l-lysine-alginate nationallicence ECM : Extracellular matrix nationallicence EGF : Epidermal growth factor nationallicence ERα : Estrogen receptor alpha nationallicence HMF : Human mammary fibroblasts nationallicence hMSC : Human mesenchymal stem cell nationallicence HTS : High-throughput screening nationallicence IFAST : Immiscible phase filtration assisted by surface tension nationallicence iPS : Inducible pluripotent stem nationallicence MCS : Multicellular spheroids nationallicence PCL : Poly(ε-caprolactone) nationallicence PDLLA : Poly(d,l-lactic acid) nationallicence PDMS : Poly(dimethylsiloxane) nationallicence PEG : Poly(ethylene glycol) nationallicence PEGDA : Poly(ethylene glycol) diacrylate nationallicence PGA : Poly(glycolic acid) nationallicence PLA : Poly(lactic acid) nationallicence PLCA : Polymer live cell array nationallicence PLGA : Poly(d,l-lactic-co-glycolic acid) nationallicence PLLA : Poly(l-lactic acid) nationallicence Poly-HEMA : Poly(2-hydroxyethyl methacrylate) nationallicence PS : Polystyrene nationallicence RGDS : Arg-Gly-Asp-Ser nationallicence VEGF : Vascular endothelial growth factor nationallicence Montanez-Sauri Sara Materials Science Program, University of Wisconsin-Madison, Madison, WI, USA aut Beebe David Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA aut Sung Kyung Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA aut Cellular and Molecular Life Sciences Springer Basel 72/2(2015-01-01), 237-249 1420-682X 72:2<237 2015 72 18 https://doi.org/10.1007/s00018-014-1738-5 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1738-5 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Montanez-Sauri Sara Materials Science Program, University of Wisconsin-Madison, Madison, WI, USA aut NATIONALLICENCE 700 1- Beebe David Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA aut NATIONALLICENCE 700 1- Sung Kyung Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/2(2015-01-01), 237-249 1420-682X 72:2<237 2015 72 18