caa a22 4500 606177132 CHVBK 20210128100750.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00018-014-1669-1 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1669-1 Neuraminidase-producing oral mitis group streptococci potentially contribute to influenza viral infection and reduction in antiviral efficacy of zanamivir [Elektronische Daten] [Noriaki Kamio, Kenichi Imai, Kazufumi Shimizu, Marni Cueno, Muneaki Tamura, Yuko Saito, Kuniyasu Ochiai] Influenza is a serious respiratory disease among immunocompromised individuals, such as the elderly, and its prevention is an urgent social issue. Influenza viruses rely on neuraminidase (NA) activity to release progeny viruses from infected cells and spreading the infection. NA is, therefore, an important target of anti-influenza drugs. A causal relationship between bacteria and influenza virus infection has not yet been established, however, a positive correlation between them has been reported. Thus, in this study, we examined the biological effects of oral mitis group streptococci, which are predominant constituents of human oral florae, on the release of influenza viruses. Among them, Streptococcus oralis ATCC 10557 and Streptococcus mitis ATCC 6249 were found to exhibit NA activity and their culture supernatants promoted the release of influenza virus and cell-to-cell spread of the infection. In addition, culture supernatants of these NA-producing oral bacteria increased viral M1 protein expression levels and cellular ERK activation. These effects were not observed with culture supernatants of Streptococcus sanguinis ATCC 10556 which lacks the ability to produce NA. Although the NA inhibitor zanamivir suppressed the release of progeny viruses from the infected cells, the viral release was restored upon the addition of culture supernatants of NA-producing S. oralis ATCC 10557 or S. mitis ATCC 6249. These findings suggest that an increase in the number of NA-producing oral bacteria could elevate the risk of and exacerbate the influenza infection, hampering the efficacy of viral NA inhibitor drugs. Springer Basel, 2014 Influenza virus nationallicence Oral bacteria nationallicence Neuraminidase nationallicence Zanamivir nationallicence Kamio Noriaki Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Imai Kenichi Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Shimizu Kazufumi Division of Microbiology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, 650-0017, Kobe, Japan aut Cueno Marni Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Tamura Muneaki Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Saito Yuko Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Ochiai Kuniyasu Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut Cellular and Molecular Life Sciences Springer Basel 72/2(2015-01-01), 357-366 1420-682X 72:2<357 2015 72 18 https://doi.org/10.1007/s00018-014-1669-1 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 research-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1669-1 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kamio Noriaki Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 700 1- Imai Kenichi Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 700 1- Shimizu Kazufumi Division of Microbiology, Center for Infectious Diseases, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, 650-0017, Kobe, Japan aut NATIONALLICENCE 700 1- Cueno Marni Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 700 1- Tamura Muneaki Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 700 1- Saito Yuko Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 700 1- Ochiai Kuniyasu Department of Microbiology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, 101-8310, Tokyo, Japan aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/2(2015-01-01), 357-366 1420-682X 72:2<357 2015 72 18