caa a22 4500 606177175 CHVBK 20210128100750.0 cr unu---uuuuu 210128e20150101xx s 000 0 eng 10.1007/s00018-014-1734-9 doi (NATIONALLICENCE)springer-10.1007/s00018-014-1734-9 Multi-layered global gene regulation in mouse embryonic stem cells [Elektronische Daten] [Samuel Beck, Bum-Kyu Lee, Jonghwan Kim] Embryonic stem (ES) cells derived from the inner cell mass of developing embryos have tremendous potential in regenerative medicine due to their unique properties: ES cells can be maintained for a prolonged time without changes in their cellular characteristics in vitro (self-renewal), while sustaining the capacity to give rise to all cell types of adult organisms (pluripotency). In addition to the development of protocols to manipulate ES cells for therapeutic applications, understanding how such unique properties are maintained has been one of the key questions in stem cell research. During the past decade, advances in high-throughput technologies have enabled us to systematically monitor multiple layers of gene regulatory mechanisms in ES cells. In this review, we briefly summarize recent findings on global gene regulatory modes in ES cells, mainly focusing on the regulatory factors responsible for transcriptional and epigenetic regulations as well as their modular regulatory patterns throughout the genome. The Author(s), 2014 Transcriptional regulation nationallicence Cis -regulatory element nationallicence Enhancer nationallicence Promoter nationallicence Core pluripotency factors nationallicence MYC class DNA binding protein (DBPs) nationallicence Polycomb repressive complex (PRC) nationallicence Co-occupancy nationallicence Epigenetic regulation nationallicence Histone modification nationallicence Histone modifiers nationallicence DNA methylation nationallicence Chromatin remodeler nationallicence High-order chromosomal structure nationallicence Long-range chromosomal interaction nationallicence Chromosomal territory nationallicence Modular transcriptional regulation nationallicence Protein-protein interaction (PPI) nationallicence Protein-DNA interaction (PDI) nationallicence ES cell : Embryonic stem cell nationallicence ICM : Inner cell mass nationallicence DBP : DNA-binding proteins nationallicence TF : Transcription factor nationallicence TE : Trophectoderm nationallicence LIF : Leukemia inhibitory factor nationallicence ChIP : Chromatin immunoprecipitation nationallicence PET : Paired-end tag nationallicence ChIP-seq : Chromatin immunoprecipitation followed by massive-parallel sequencing nationallicence PPI : Protein-protein interaction nationallicence PDI : Protein-DNA interaction nationallicence NODE : Nanog and Oct4-associated deacetylase nationallicence NuA4 : Nucleosome acetyltransferase of H4 nationallicence CGI : CpG island nationallicence DNMT : DNA methyltransferase nationallicence Mbd : Methyl-binding domain containing protein nationallicence NuRD : Nucleosome remodeling deacetylase nationallicence DKO : Double knockout nationallicence H3K36me3 : Histone H3 lysine 36 trimethylation nationallicence H3K4me1 : Histone H3 lysine 4 monomethylation nationallicence H3K4me3 : Histone H3 lysine 4 trimethylation nationallicence H3K27me3 : Histone H3 lysine 27 trimethylation nationallicence H3K27ac : Histone H3 lysine 27 acetylation nationallicence H2AK119ub : Histone H2A lysine 119 ubiquitination nationallicence EB : Embryoid body nationallicence CHD : Chromodomain helicase DNA-binding nationallicence FISH : Fluorescence in situ hybridization nationallicence 3C : Chromosome conformation capture nationallicence 4C : Circular chromosome conformation capture nationallicence 5C : Carbon-copy chromosome conformation capture nationallicence ChIA-PET : Chromatin interaction analysis by paired-end tag sequencing nationallicence PolII : RNA polymerase II nationallicence Beck Samuel Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, The University of Texas at Austin, 78712, Austin, TX, USA aut Lee Bum-Kyu Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, The University of Texas at Austin, 78712, Austin, TX, USA aut Kim Jonghwan Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, The University of Texas at Austin, 78712, Austin, TX, USA aut Cellular and Molecular Life Sciences Springer Basel 72/2(2015-01-01), 199-216 1420-682X 72:2<199 2015 72 18 https://doi.org/10.1007/s00018-014-1734-9 text/html Onlinezugriff via DOI BK010053 XK010053 XK010000 Metadata rights reserved Springer special CC-BY-NC licence nationallicence 1 review-article jats NATIONALLICENCE NATIONALLICENCE NL-springer NATIONALLICENCE 856 40 https://doi.org/10.1007/s00018-014-1734-9 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Beck Samuel Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, The University of Texas at Austin, 78712, Austin, TX, USA aut NATIONALLICENCE 700 1- Lee Bum-Kyu Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, The University of Texas at Austin, 78712, Austin, TX, USA aut NATIONALLICENCE 700 1- Kim Jonghwan Department of Molecular Biosciences, Institute for Cellular and Molecular Biology, Center for Systems and Synthetic Biology, The University of Texas at Austin, 78712, Austin, TX, USA aut NATIONALLICENCE 773 0- Cellular and Molecular Life Sciences Springer Basel 72/2(2015-01-01), 199-216 1420-682X 72:2<199 2015 72 18